Requirement for Bbp1p in the proper mitotic functions of Cdc5p in Saccharomyces cerevisiae

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dc.contributor.authorC J Park-
dc.contributor.authorS G Song-
dc.contributor.authorT H Giddings-
dc.contributor.authorHyeon Su Ro-
dc.contributor.authorK Sakchaisri-
dc.contributor.authorJ E Park-
dc.contributor.authorY S Seong-
dc.contributor.authorM Winey-
dc.contributor.authorK S Lee-
dc.date.accessioned2017-04-19T09:00:58Z-
dc.date.available2017-04-19T09:00:58Z-
dc.date.issued2004-
dc.identifier.issn1059-1524-
dc.identifier.uri10.1091/mbc.E03-07-0461ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6448-
dc.description.abstractThe polo-box domain of the budding yeast polo kinase Cdc5p plays an essential role for targeting the catalytic activity of Cdc5p to spindle pole bodies (SPBs) and cytokinetic neck-filaments. Here, we report the isolation of Bbp1p as a polo-box interacting protein by a yeast two-hybrid screen. Bbp1p localizes to the periphery of the central plaque of the SPB and plays an important role in SPB duplication. Similarly, Cdc5p localized to the cytoplasmic periphery of the SPB. In vitro binding studies showed that Cdc5p interacted with the N-terminal domain of Bbp1p (Bbp1pΔC), but apparently not with Mps2p, a component shown to form a stable complex with Bbp1p. In addition, Bbp1p, but likely not Mps2p, was required for proper localization of Cdc5p to the SPB. The C-terminal coiled-coil domain of Bbp1p (Bbp1p 243-385), which is crucial for both the homodimerization and the SPB localization, could target the localization-defective Cdc5pΔC to the SPB and induce the release of Cdc14p from the nucleolus. Consistent with this observation, expression of CDC5ΔC-BBP1243-385 under CDC5 promoter control partially complemented the cdc5Δ defect. These data suggest that Bbp1pΔC interacts with the polo-box domain of Cdc5p, and this interaction is critical for the subcellular localization and mitotic functions of Cdc5p.-
dc.publisherAmer Soc Cell Biology-
dc.titleRequirement for Bbp1p in the proper mitotic functions of Cdc5p in Saccharomyces cerevisiae-
dc.title.alternativeRequirement for Bbp1p in the proper mitotic functions of Cdc5p in Saccharomyces cerevisiae-
dc.typeArticle-
dc.citation.titleMolecular Biology of Cell-
dc.citation.number4-
dc.citation.endPage1723-
dc.citation.startPage1711-
dc.citation.volume15-
dc.contributor.affiliatedAuthorHyeon Su Ro-
dc.contributor.alternativeName박종-
dc.contributor.alternativeName송석길-
dc.contributor.alternativeNameGiddings-
dc.contributor.alternativeName노현수-
dc.contributor.alternativeNameSakchaisri-
dc.contributor.alternativeName박정은-
dc.contributor.alternativeName성연순-
dc.contributor.alternativeNameWiney-
dc.contributor.alternativeName이경-
dc.identifier.bibliographicCitationMolecular Biology of Cell, vol. 15, no. 4, pp. 1711-1723-
dc.identifier.doi10.1091/mbc.E03-07-0461-
dc.description.journalClassY-
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