Purification and characterization of six fibrinolytic serine-proteases from earthworm Lumbricus rubellus

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dc.contributor.authorI H Cho-
dc.contributor.authorEui Sung Choi-
dc.contributor.authorH G Lim-
dc.contributor.authorH H Lee-
dc.date.accessioned2017-04-19T09:00:59Z-
dc.date.available2017-04-19T09:00:59Z-
dc.date.issued2004-
dc.identifier.issn1225-8687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6457-
dc.description.abstractThe six lumbrokinase fractions (F1 to F6) with fibrinolytic activities were purified from earthworm Lumbricus rubellus lysates using the procedures of autolysis, ammonium sulfate fractionation, and column chromatography. The proteolytic activities on the casein substrate of the six iso-enzymes ranged from 11.3 to 167.5 unit/mg with the rank activity orders of F2 > F1 > F5 > F6 > F3 > F4. The fibrinolytic activities of the six fractions on the fibrin plates ranged from 20.8 to 207.2 unit/mg with rank orders of F6 > F2 > F5 > F3 > F1 > F4. The molecular weights of each iso-enzyme, as estimated by SDS-PAGE, were 24.6 (F1), 26.8 (F2), 28.2 (F3), 25.4 (F4), 33.1 (F5), and 33.0 kDa (F6), respectively. The plasminogen was activated into plasmin by the enzymes. The optimal temperature of the six iso-enzymes was 50°C, and the optimal pH ranged from pH 4-12. The four iso-enzymes (F1-F4) were completely inhibited by PMSF. The two enzymes (F5 and F6) were completely inhibited by aprotinin, TLCK, TPCK, SBTI, LBTI, and leupeptin. The N-terminal amino acid (aa) sequences of the first 20 to 22 residues of each fraction had high homology. All six iso-enzymes had identical aa residues 2-3 and 13-15. The N-terminal 21-22 aa sequences of the F2, F3, and F4 iso-enzymes were almost the same. The N-terminal aa sequences of F5 and F6 were identical.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titlePurification and characterization of six fibrinolytic serine-proteases from earthworm Lumbricus rubellus-
dc.title.alternativePurification and characterization of six fibrinolytic serine-proteases from earthworm Lumbricus rubellus-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number2-
dc.citation.endPage205-
dc.citation.startPage199-
dc.citation.volume37-
dc.contributor.affiliatedAuthorEui Sung Choi-
dc.contributor.alternativeName조일환-
dc.contributor.alternativeName최의성-
dc.contributor.alternativeName임헌길-
dc.contributor.alternativeName이형환-
dc.identifier.bibliographicCitationBMB Reports, vol. 37, no. 2, pp. 199-205-
dc.identifier.doi10.5483/bmbrep.2004.37.2.199-
dc.subject.keywordfibrin-
dc.subject.keywordlumbricus rubellus-
dc.subject.keywordlumbrokinase-
dc.subject.localFibrin-
dc.subject.localfibrin-
dc.subject.locallumbricus rubellus-
dc.subject.locallumbrokinase-
dc.description.journalClassY-
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Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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