Detection of Nocardia sp. H17-1 by PCR during bioremediation of crude oil-contaminated soil = 원유 오염토양의 bioremediation 과정 동안 PCR을 이용한 Nocardia sp. H17-1의 검출

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Title
Detection of Nocardia sp. H17-1 by PCR during bioremediation of crude oil-contaminated soil = 원유 오염토양의 bioremediation 과정 동안 PCR을 이용한 Nocardia sp. H17-1의 검출
Author(s)
Kyung Hwa Baek; Young Ki Lee; I S Lee; Hee-Mock Oh; Byung Dae Yoon; Hee Sik Kim
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 32, no. 1, pp. 91-95
Publication Year
2004
Abstract
For the detection of the oil-degrading bacterium, Nocardia sp. H17-1, inoculated during the bioremediation of oil-contaminated soil, a species-specific primer was constructed based on the 16S rDNA sequence of this strain. Two forward primers and two reverse primers were designed and tested against both closely and distantly related bacterial strains. All the primers designed were specific to the Nocardia sp. H17-1. Particularly, primer sets NH169F-NH972R and NH575F-NH972R could be used to detect 50 fg of template DNA and 1.2×104 CFU/g of sandy soil. These two PCR primer sets successfully detected the H17-1 strain in the oil-contaminated soil samples containing heterogeneous DNA. We also conformed the primer specificity by restriction-enzyme cleavage of the PCR products and denaturing gradient gel electrophoresis.
Keyword
denaturing gradient gel electrophoresis (DGGE)detectionl6S rDNAnocardia sp.PCRspecies-specific primer
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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