Cloning and functional characterization of the SUR2/SYR2 gene encoding sphinganine hydroxylase in Pichia ciferrii

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dc.contributor.authorJung Hoon Bae-
dc.contributor.authorJung Hoon Sohn-
dc.contributor.authorC S Park-
dc.contributor.authorJ S Rhee-
dc.contributor.authorEui Sung Choi-
dc.date.accessioned2017-04-19T09:01:01Z-
dc.date.available2017-04-19T09:01:01Z-
dc.date.issued2004-
dc.identifier.issn0749-503X-
dc.identifier.uri10.1002/yea.1082ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6470-
dc.description.abstractSaccharomyces cerevisiae sphinganine C4-hydroxylase encoded by the SUR2 gene catalyses the conversion of sphinganine to phytosphingosine. We isolated the SUR2 gene from Pichia ciferiii using nucleotide sequence homology to S. cerevisiae SUR2 to study hydroxylation of sphinganine in the sphingoid base overproducing yeast P. ciferrii. A positive clone was confirmed by nucleotide sequencing. A syringomycin-E resistance phenotype of a S. cerevisiae sur2-null mutant was complemented by expression of the cloned P. ciferrii SUR2 gene. Restoration of phytosphingosine production in the complemented strain was also confirmed, indicating that the cloned gene is a functional homologue of S. cerevisiae SUR2. The nucleotide sequence of the P. ciferrii SUR2 gene was submitted to GenBank under Accession No. AY151276.-
dc.publisherWiley-
dc.titleCloning and functional characterization of the SUR2/SYR2 gene encoding sphinganine hydroxylase in Pichia ciferrii-
dc.title.alternativeCloning and functional characterization of the SUR2/SYR2 gene encoding sphinganine hydroxylase in Pichia ciferrii-
dc.typeArticle-
dc.citation.titleYeast-
dc.citation.number5-
dc.citation.endPage443-
dc.citation.startPage437-
dc.citation.volume21-
dc.contributor.affiliatedAuthorJung Hoon Bae-
dc.contributor.affiliatedAuthorJung Hoon Sohn-
dc.contributor.affiliatedAuthorEui Sung Choi-
dc.contributor.alternativeName배정훈-
dc.contributor.alternativeName손정훈-
dc.contributor.alternativeName박창서-
dc.contributor.alternativeName이준식-
dc.contributor.alternativeName최의성-
dc.identifier.bibliographicCitationYeast, vol. 21, no. 5, pp. 437-443-
dc.identifier.doi10.1002/yea.1082-
dc.subject.keywordHydroxylation-
dc.subject.keywordPichia ciferrii-
dc.subject.keywordSUr2-
dc.subject.keywordSyringomycin-E-
dc.subject.localHydroxylation-
dc.subject.localhydroxylation-
dc.subject.localPichia ciferrii-
dc.subject.localSUr2-
dc.subject.localSyringomycin-E-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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