A method of high frequency virus-induced gene silencing in chili pepper(Capsicum annuum L. cv. Bukang)

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Title
A method of high frequency virus-induced gene silencing in chili pepper(Capsicum annuum L. cv. Bukang)
Author(s)
Eunsook Chung; Eun Soo Seong; Yeoung Cheol Kim; Eun Joo Chung; Sang-Keun Oh; Sanghyeob Lee; Jeong Mee Park; Young Hee Joung; Do Il Choi
Bibliographic Citation
Molecules and Cells, vol. 17, no. 2, pp. 377-380
Publication Year
2004
Abstract
Using a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system, expression of phytogene desaturase (PDS) and ribulose-1,5-bisphosphate carboxylase small-subuit (rbcS) genes was suppressed in Nicotiana benthamiana and pepper plants (Capsicum annuum L. cv. Bukang). The silenced phenotypes of pale yellow (rbcS), and photobleached leaves (PDS), were invariably obvious 2 weeks after inoculation with the TRV-based vector. In a parallel experiment, the same set of genes was silenced in N. benthamiana and yielded identical phenotypes to pepper 1 week after inoculation. Northern blot analyses showed that the endogenous levels of CarbcS and CaPDS transcripts were dramatically reduced in the silenced leaf tissues. These observations confirm that the silenced phenotype is closely correlated with the pattern of tissue expression. To our knowledge, this is the first high frequency VIGS method in pepper plants. It should provide a tool for large-scale gene silencing studies in pepper functional genomics.
Keyword
C. annuumN. benthamianapeppersuppressionTRVVIGS
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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