Plant regeneration from immature zygotic embryo-derived embryogenic calluses and cell suspension cultures of Catharanthus roseus

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Title
Plant regeneration from immature zygotic embryo-derived embryogenic calluses and cell suspension cultures of Catharanthus roseus
Author(s)
Suk Weon Kim; D S In; P S Choi; Jang Ryol Liu
Bibliographic Citation
Plant Cell Tissue and Organ Culture, vol. 76, no. 2, pp. 131-135
Publication Year
2004
Abstract
Culture conditions for plant regeneration in immature zygotic embryo-derived embryogenic cell suspension cultures of Catharanthus roseus (Madagascar periwinkle) 'Little Bright Eye' are described. Immature zygotic embryos formed off-white, friable calluses at a frequency of 20% on Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D) after 8 weeks of culture. After a second subculture using MS basal medium at 4-week intervals, off-white friable calluses formed a small quantity of yellowish, compact embryogenic calluses. Upon transfer to MS basal medium, embryogenic calluses gave rise to numerous somatic embryos. Cell suspension cultures were established with embryogenic calluses using liquid MS medium supplemented with 4.52 μM 2,4-D. Embryogenic cell clumps from cell suspension cultures developed into plantlets at a frequency of 56.7% when plated onto MS basal medium. Plantlets were transplanted to potting soil and grown to maturity in a growth chamber.
Keyword
ApocynaceaeImmature zygotic embryoMadagascar periwinkleSomatic embryoSomatic embryogenesis
ISSN
0167-6857
Publisher
Springer
DOI
http://dx.doi.org/10.1023/B:TICU.0000007254.51387.7f
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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