Nano-scale proteomics approach using two-dimensional fibrin zymography combined with fluorescent SYPRO Ruby dye

Cited 19 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorNack Shick Choi-
dc.contributor.authorKi Hyun Yoo-
dc.contributor.authorKab Seog Yoon-
dc.contributor.authorP J Maeng-
dc.contributor.authorSeung Ho Kim-
dc.date.accessioned2017-04-19T09:01:06Z-
dc.date.available2017-04-19T09:01:06Z-
dc.date.issued2004-
dc.identifier.issn1225-8687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6501-
dc.description.abstractIn general, a SYPRO Ruby dye is well known as a sensitive fluorescence-based method for detecting proteins by one-or two-dimensional SDS-PAGE (1-DE or 2-DE). Based on the SYPRO Ruby dye system, the combined two-dimensional fibrin zymography (2-D FZ) with SYPRO Ruby staining was newly developed to identify the Bacillus sp. proteases. Namely, complex protein mixtures from Bacillus sp. DJ-4, which were screened from Doen-Jang (Korean traditional fermented food), showed activity on the zymogram gel. The gel spots on the SYPRO Ruby gel, which corresponded to the active spots showing on the 2-D FZ gel, were analyzed by a matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometric analysis. Five intracellular fibrinolytic enzymes of Bacillus sp. DJ-4 were detected through 2-D FZ. The gel spots on the SYPRO Ruby dye stained 2-D gel corresponding to 2-D FZ were then analyzed by MALID-TOF MS. Three of the five gel spots proved to be quite similar to the ATP-dependent protease, extracellular neutral metalloprotease, and protease of Bacillus subtilis. Also, the extracellular proteases of Bacillus sp. DJ-4 employing this combined system were identified on three gels (e.g., casein, fibrin, and gelatin) and the proteolytic maps were established. This combined system of 2-D zymography and SYPRO Ruby dye should be useful for searching the specific protease from complex protein mixtures of many other sources (e.g., yeast and cancer cell lines).-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleNano-scale proteomics approach using two-dimensional fibrin zymography combined with fluorescent SYPRO Ruby dye-
dc.title.alternativeNano-scale proteomics approach using two-dimensional fibrin zymography combined with fluorescent SYPRO Ruby dye-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number3-
dc.citation.endPage303-
dc.citation.startPage298-
dc.citation.volume37-
dc.contributor.affiliatedAuthorNack Shick Choi-
dc.contributor.affiliatedAuthorKi Hyun Yoo-
dc.contributor.affiliatedAuthorKab Seog Yoon-
dc.contributor.affiliatedAuthorSeung Ho Kim-
dc.contributor.alternativeName최낙식-
dc.contributor.alternativeName유기현-
dc.contributor.alternativeName윤갑석-
dc.contributor.alternativeName맹필재-
dc.contributor.alternativeName김승호-
dc.identifier.bibliographicCitationBMB Reports, vol. 37, no. 3, pp. 298-303-
dc.identifier.doi10.5483/bmbrep.2004.37.3.298-
dc.subject.keywordbacillus-
dc.subject.keywordproteomics-
dc.subject.keywordSYPRO ruby-
dc.subject.keywordtwo-dimension-
dc.subject.keywordzymography-
dc.subject.localBacillus-
dc.subject.localbacillus-
dc.subject.localproteomics-
dc.subject.localSYPRO ruby-
dc.subject.localtwo-dimension-
dc.subject.localZymography-
dc.subject.localzymography-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.