Sequence-based approach to finding functional lipases from microbial genome databases

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dc.contributor.authorH K Kim-
dc.contributor.authorYeo Jin Jung-
dc.contributor.authorWon Chan Choi-
dc.contributor.authorHyoung Seok Ryu-
dc.contributor.authorTae Kwang Oh-
dc.contributor.authorJung-Kee Lee-
dc.date.accessioned2017-04-19T09:01:07Z-
dc.date.available2017-04-19T09:01:07Z-
dc.date.issued2004-
dc.identifier.issn0378-1097-
dc.identifier.uri10.1016/j.femsle.2004.05.004ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6505-
dc.description.abstractA sequence-based approach was used to retrieve functional lipases from microbial genome databases. Many novel genes assigned as putative lipases were tested using the criteria of the typical lipase sequence rule, based on a consensus sequence of a catalytic triad (Ser, Asp, His) and oxyanion hole sequence (HG). To obtain the lipase genes satisfying the sequence rule, PCR cloning was performed, while the lipase activities were tested using a tributyrin/tricaprylin plate and p-nitrophenyl caproate. Among nine putative lipases from four strains, five functional lipolytic proteins were obtained from Archaeoglobus fulgidus, Deinococcus radiodurans, and Agrobacterium tumefaciens. All five lipases exhibited a relatively low sequence similarity (less than 26.7%) with known lipases and turned out to belong to different lipase families. Accordingly, the current results indicate that the proposed strategic approach based on the microbial genome is an efficient and rapid method for finding novel and functional lipases.-
dc.publisherOxford Univ Press-
dc.titleSequence-based approach to finding functional lipases from microbial genome databases-
dc.title.alternativeSequence-based approach to finding functional lipases from microbial genome databases-
dc.typeArticle-
dc.citation.titleFEMS Microbiology Letters-
dc.citation.number2-
dc.citation.endPage355-
dc.citation.startPage349-
dc.citation.volume235-
dc.contributor.affiliatedAuthorYeo Jin Jung-
dc.contributor.affiliatedAuthorWon Chan Choi-
dc.contributor.affiliatedAuthorHyoung Seok Ryu-
dc.contributor.affiliatedAuthorTae Kwang Oh-
dc.contributor.affiliatedAuthorJung-Kee Lee-
dc.contributor.alternativeName김형권-
dc.contributor.alternativeName정여진-
dc.contributor.alternativeName최원찬-
dc.contributor.alternativeName류형석-
dc.contributor.alternativeName오태광-
dc.contributor.alternativeName이정기-
dc.identifier.bibliographicCitationFEMS Microbiology Letters, vol. 235, no. 2, pp. 349-355-
dc.identifier.doi10.1016/j.femsle.2004.05.004-
dc.subject.keywordagrobacterium tumefaciens-
dc.subject.keywordarchaeoglobus fulgidus-
dc.subject.keyworddeinococcus radiodurans-
dc.subject.keywordlipase-
dc.subject.keywordmicrobial genome database-
dc.subject.localagrobacterium tumefaciens-
dc.subject.localAgrobacterium tumefaciens-
dc.subject.localarchaeoglobus fulgidus-
dc.subject.localDeinococcus radiodurans-
dc.subject.localdeinococcus radiodurans-
dc.subject.locallipase-
dc.subject.localLipase-
dc.subject.localmicrobial genome database-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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