Evaluation of cellulose-binding domain fused to a lipase for the lipase immobilization

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dc.contributor.authorS P Hwang-
dc.contributor.authorJungoh Ahn-
dc.contributor.authorS M Lee-
dc.contributor.authorT G Lee-
dc.contributor.authorS J Haam-
dc.contributor.authorK T Lee-
dc.contributor.authorI S Ahn-
dc.contributor.authorJoon Ki Jung-
dc.date.accessioned2017-04-19T09:01:08Z-
dc.date.available2017-04-19T09:01:08Z-
dc.date.issued2004-
dc.identifier.issn0141-5492-
dc.identifier.uri10.1023/B:BILE.0000021964.69500.6fko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6514-
dc.description.abstractA cellulose-binding domain (CBD) fragment of a cellulase gene of Trichoderma hazianum was fused to a lipase gene of Bacillus stearothermophilus L1 to make a gene cluster for CBD-BSL lipase. The specific activity of CBD-BSL lipase for oil hydrolysis increased by 33% after being immobilized on Avicel (microcrystalline cellulose), whereas those of CBD-BSL lipase and BSL lipase decreased by 16% and 54%, respectively, after being immobilized on silica gel. Although the loss of activity of an enzyme immobilized by adsorption has been reported previously, the loss of activity of the CBD-BSL lipase immobilized on Avicel was less than 3% after 12 h due to the irreversible binding of CBD to Avicel.-
dc.publisherSpringer-
dc.titleEvaluation of cellulose-binding domain fused to a lipase for the lipase immobilization-
dc.title.alternativeEvaluation of cellulose-binding domain fused to a lipase for the lipase immobilization-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number7-
dc.citation.endPage605-
dc.citation.startPage603-
dc.citation.volume26-
dc.contributor.affiliatedAuthorJungoh Ahn-
dc.contributor.affiliatedAuthorJoon Ki Jung-
dc.contributor.alternativeName황상필-
dc.contributor.alternativeName안정오-
dc.contributor.alternativeName이수민-
dc.contributor.alternativeName이태규-
dc.contributor.alternativeName함승주-
dc.contributor.alternativeName이강택-
dc.contributor.alternativeName안익성-
dc.contributor.alternativeName정준기-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 26, no. 7, pp. 603-605-
dc.identifier.doi10.1023/B:BILE.0000021964.69500.6f-
dc.subject.keywordAdsorption-
dc.subject.keywordAvicel-
dc.subject.keywordCellulose-binding domain-
dc.subject.keywordImmobilization-
dc.subject.keywordLipase-
dc.subject.localadsorption-
dc.subject.localAdsorption-
dc.subject.localAvicel-
dc.subject.localCellulose-binding domain (CBD)-
dc.subject.localCellulose-binding domain-
dc.subject.localcellulose-binding domain-
dc.subject.localimmobilization-
dc.subject.localImmobilization-
dc.subject.locallipase-
dc.subject.localLipase-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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