Characterization of a monoclonal antibody specific to human Siah-1 interacting protein = 인체 SIP 단백질에 특이적인 단일클론 항체의 특성

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Title
Characterization of a monoclonal antibody specific to human Siah-1 interacting protein = 인체 SIP 단백질에 특이적인 단일클론 항체의 특성
Author(s)
Sun Young Yoon; Joung Hyuck Joo; J H Kim; Ho Bum Kang; Jin Sook Kim; Y H Lee; Dur Han Kwon; C N Kim; In Seong Choe; Jae Wha Kim
Bibliographic Citation
Immune Network, vol. 4, no. 1, pp. 23-30
Publication Year
2004
Abstract
Background: A human orthologue of mouse S100A6-binding protein (CacyBP), Siah-1-interacting protein (SIP) had been shown to be a component of novel ubiquitinylation pathway regulating β-catenin degradation. The role of the protein seems to be important in cell proliferation and cancer evolution but the expression pattern of SIP in actively dividing cancer tissues has not been known. For the elucidation of the role of SIP protein in carcinogenesis, it is essential to produce monoclonal antibodies specific to the protein. Methods: cDNA sequence coding for ORF region of human SIP gene was amplified and cloned into an expression vector to produce His-tag fusion protein. Recombinant SIP protein and monoclonal antibody to the protein were produced. The N-terminal specificity of anti-SIP monoclonal antibody was conformed by immunoblot analysis and enzyme linked immunosorbent assay (ELISA). To study the relation between SIP and colon carcinogenesis, the presence of SIP protein in colon carcinoma tissues was visualized by immunostaining using the monoclonal antibody produced in this study. Results: His-tag-SIP (NSIP) recombinant protein was produced and purified. A monoclonal antibody (Korea patent pending; #2003-45296) to the protein was produced and employed to analyze the expression pattern of SIP in colon carcinoma tissues. Conclusion: The data suggested that anti-SIP monoclonal antibody produced here was valuable for the diagnosis of colon carcinoma and elucidation of the mechanism of colon carcinogenesis.
Keyword
siah-1 interacting protein (SIP)S100A6monoclonal antibodycolorectal cancer
ISSN
I000-0135
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4110/in.2004.4.1.23
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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