Identification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699

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dc.contributor.authorS K Lee-
dc.contributor.authorC Y Choi-
dc.contributor.authorJong Seog Ahn-
dc.contributor.authorJ Y Cho-
dc.contributor.authorC S Park-
dc.contributor.authorY J Yoon-
dc.date.accessioned2017-04-19T09:01:12Z-
dc.date.available2017-04-19T09:01:12Z-
dc.date.issued2004-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6534-
dc.description.abstractIn analyzing the region of the Amycolatopsis mediterranei S699 chromosome responsible for the biosynthesis of the ansamycin antibiotic rifamycin, we identified a gene, designated orf0, which is located immediately upstream of the rifamycin polyketide synthase (PKS). Orf0 encodes a protein, on the basis of sequence-comparative analysis, that is similar to several cytochrome P450 monooxygenases from different sources. The rifamycin producer, A. mediterranei, predominantly produces rifamycin B from its macrocyclic intermediate, proansamycin X, through dehydrogenation and hydroxylation steps. However, an A. mediterranei strain, deleted in orf0 by gene replacement, no longer produced rifamycin B. Furthermore, a versatile replicative vector in A. mediterranei was constructed and rifamycin B production was restored in a complementation experiment of orf0 using this novel vector. These consecutive results verified that the Orf0 protein, which is a P450 hydroxylase, is required for the production of rifamycin B in A. mediterranei.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleIdentification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699-
dc.title.alternativeIdentification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number2-
dc.citation.endPage362-
dc.citation.startPage356-
dc.citation.volume14-
dc.contributor.affiliatedAuthorJong Seog Ahn-
dc.contributor.alternativeName이상길-
dc.contributor.alternativeName최차용-
dc.contributor.alternativeName안종석-
dc.contributor.alternativeName조재용-
dc.contributor.alternativeName박천석-
dc.contributor.alternativeName윤여준-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 14, no. 2, pp. 356-362-
dc.subject.keywordamycolatopsis mediterranei-
dc.subject.keywordP450 monooxygenase-
dc.subject.keywordproansamycin X-
dc.subject.keywordreplication vector-
dc.subject.keywordrifamycin-
dc.subject.localamycolatopsis mediterranei-
dc.subject.localP450 monooxygenase-
dc.subject.localproansamycin X-
dc.subject.localreplication vector-
dc.subject.localrifamycin-
dc.subject.localRifamycin-
dc.description.journalClassY-
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Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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