DC Field | Value | Language |
---|---|---|
dc.contributor.author | S K Lee | - |
dc.contributor.author | C Y Choi | - |
dc.contributor.author | Jong Seog Ahn | - |
dc.contributor.author | J Y Cho | - |
dc.contributor.author | C S Park | - |
dc.contributor.author | Y J Yoon | - |
dc.date.accessioned | 2017-04-19T09:01:12Z | - |
dc.date.available | 2017-04-19T09:01:12Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6534 | - |
dc.description.abstract | In analyzing the region of the Amycolatopsis mediterranei S699 chromosome responsible for the biosynthesis of the ansamycin antibiotic rifamycin, we identified a gene, designated orf0, which is located immediately upstream of the rifamycin polyketide synthase (PKS). Orf0 encodes a protein, on the basis of sequence-comparative analysis, that is similar to several cytochrome P450 monooxygenases from different sources. The rifamycin producer, A. mediterranei, predominantly produces rifamycin B from its macrocyclic intermediate, proansamycin X, through dehydrogenation and hydroxylation steps. However, an A. mediterranei strain, deleted in orf0 by gene replacement, no longer produced rifamycin B. Furthermore, a versatile replicative vector in A. mediterranei was constructed and rifamycin B production was restored in a complementation experiment of orf0 using this novel vector. These consecutive results verified that the Orf0 protein, which is a P450 hydroxylase, is required for the production of rifamycin B in A. mediterranei. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Identification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699 | - |
dc.title.alternative | Identification of a cytochrome P450 hydroxylase gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699 | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 362 | - |
dc.citation.startPage | 356 | - |
dc.citation.volume | 14 | - |
dc.contributor.affiliatedAuthor | Jong Seog Ahn | - |
dc.contributor.alternativeName | 이상길 | - |
dc.contributor.alternativeName | 최차용 | - |
dc.contributor.alternativeName | 안종석 | - |
dc.contributor.alternativeName | 조재용 | - |
dc.contributor.alternativeName | 박천석 | - |
dc.contributor.alternativeName | 윤여준 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 14, no. 2, pp. 356-362 | - |
dc.subject.keyword | amycolatopsis mediterranei | - |
dc.subject.keyword | P450 monooxygenase | - |
dc.subject.keyword | proansamycin X | - |
dc.subject.keyword | replication vector | - |
dc.subject.keyword | rifamycin | - |
dc.subject.local | amycolatopsis mediterranei | - |
dc.subject.local | P450 monooxygenase | - |
dc.subject.local | proansamycin X | - |
dc.subject.local | replication vector | - |
dc.subject.local | rifamycin | - |
dc.subject.local | Rifamycin | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.