Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus

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Title
Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus
Author(s)
P S Choi; Y D Kim; K M Choi; H J Chung; D W Choi; Jang Ryol Liu
Bibliographic Citation
Plant Cell Reports, vol. 22, no. 11, pp. 828-831
Publication Year
2004
Abstract
Hypocotyl explants of Catharanthus roseus produced hairy roots when cultured on Murashige and Skoog (MS) basal medium after infection by Agrobacterium rhizogenes. Explants gave rise to adventitious shoots at a frequency of up to 80% when cultured on MS medium supplemented with 31.1 μM 6-benzyladenine and 5.4 μM α-naphthaleneacetic acid. There was a significant difference in the frequency of adventitious shoot formation for each hairy-root line derived from a different cultivar. Plants derived from hairy roots exhibited prolific rooting and had shortened internodes. Approximately half of the plants had wrinkled leaves and an abundant root mass with extensive lateral branching, but otherwise appeared morphologically normal. Plants with hairy roots that were derived from the cultivar Cooler Apricot developed flowers with petals that were white in the proximal region, whereas the wild-type flower petals are red. PCR and Southern blot analyses revealed that plants derived from hairy roots retained the Ri TL-DNA.
Keyword
Agrobacterium rhizogenesApocynaceaeMadagascar periwinkleTransgenic plant
ISSN
0721-7714
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00299-004-0765-3
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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