Up-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line

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dc.contributor.authorJin Koo Kim-
dc.contributor.authorKwang Dong Kim-
dc.contributor.authorE S Lee-
dc.contributor.authorJong-Seok Lim-
dc.contributor.authorHee Jun Cho-
dc.contributor.authorHyun Kyung Yoon-
dc.contributor.authorMi Young Cho-
dc.contributor.authorKyoung Eun Baek-
dc.contributor.authorYuk-Pheel Park-
dc.contributor.authorS G Paik-
dc.contributor.authorYong Kyung Choe-
dc.contributor.authorHee Gu Lee-
dc.date.accessioned2017-04-19T09:01:15Z-
dc.date.available2017-04-19T09:01:15Z-
dc.date.issued2004-
dc.identifier.issn03043835-
dc.identifier.uri10.1016/j.canlet.2004.02.021ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6553-
dc.description.abstractThe potent anti-cancer agent cis-diamminedichloroplatinum (II) (cisplatin) is currently used for treating bladder cancer. However, clinical use of this drug for long periods is often limited because of the appearance of cisplatin-resistant bladder tumor cells. We employed the method of a differential display reverse transcriptase polymerase chain reaction to identify the differentially expressed genes in the parental human bladder cancer cell line, T24 and three cisplatin-resistant cell lines. We report here that cisplatin-resistant cell lines overexpress Bcl-2 family protein Bcl-2-related gene expressed in fetal liver (Bfl-1)/A1 as compared with their parental cell. Cisplatin and γ-irradiation induced expression of Bfl-1/A1 in T24R2 cells but not in T24 cells. Among Bcl-2 family members, Bfl-1/A1 showed the most significant alteration of the expression level in resistant cells. The nuclear translocation of nuclear factor-kappaB (NF-κB) by cisplatin and γ-irradiation selectively occurred in T24R2 cells. Mitochondrial depolarization and cell death by cisplatin were also prevented in T24R2 cells. Moreover, Bfl-1/A1 inhibited cisplatin- and TNF-α-induced apoptosis in BOSC23 cells. Our findings suggest that the induction of Bfl-1/A1 by NF-κB may be important in controlling resistance to cisplatin responses in bladder tumor cells.-
dc.publisherElsevier-
dc.titleUp-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line-
dc.title.alternativeUp-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line-
dc.typeArticle-
dc.citation.titleCancer Letters-
dc.citation.number1-
dc.citation.endPage70-
dc.citation.startPage61-
dc.citation.volume212-
dc.contributor.affiliatedAuthorHee Jun Cho-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName김진구-
dc.contributor.alternativeName김광동-
dc.contributor.alternativeName이은식-
dc.contributor.alternativeName임종석-
dc.contributor.alternativeName조희준-
dc.contributor.alternativeName윤현경-
dc.contributor.alternativeName조미영-
dc.contributor.alternativeName백경은-
dc.contributor.alternativeName박육필-
dc.contributor.alternativeName백상기-
dc.contributor.alternativeName최용경-
dc.contributor.alternativeName이희구-
dc.identifier.bibliographicCitationCancer Letters, vol. 212, no. 1, pp. 61-70-
dc.identifier.doi10.1016/j.canlet.2004.02.021-
dc.subject.keywordBfl-1, Bcl-2-related gene expressed in fetal liver-
dc.subject.keywordBfl-1/A1-
dc.subject.keywordcisplatin, cis-diamminedichloroplatinum (II)-
dc.subject.keywordcisplatin-resistant bladder cancer-
dc.subject.keyworddifferential display-
dc.subject.keywordNF-κB-
dc.subject.localBfl-1, Bcl-2-related gene expressed in fetal liver-
dc.subject.localBfl-1/A1-
dc.subject.localcisplatin, cis-diamminedichloroplatinum (II)-
dc.subject.localcisplatin-resistant bladder cancer-
dc.subject.localdifferential display-
dc.subject.localDifferential display-
dc.subject.localNF-κB-
dc.subject.localNF-kB-
dc.description.journalClassY-
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Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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