Polysaccharide isolated from Poria cocos sclerotium induces NF-κB/Rel activation and iNOS expression through the activation of p38 kinase in murine macrophages

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dc.contributor.authorK Y Lee-
dc.contributor.authorH J You-
dc.contributor.authorH G Jeong-
dc.contributor.authorJong Soon Kang-
dc.contributor.authorHwan Mook Kim-
dc.contributor.authorS D Rhee-
dc.contributor.authorY J Jeon-
dc.date.accessioned2017-04-19T09:01:15Z-
dc.date.available2017-04-19T09:01:15Z-
dc.date.issued2004-
dc.identifier.issn15675769-
dc.identifier.uri10.1016/j.intimp.2004.03.014ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6555-
dc.description.abstractIn our previous studies, we showed that PCSC, a polysaccharide isolated from Poria cocos, activated macrophages to induce the translocation of NF-κB/Rel into nucleus and DNA binding to its cognate site in the promoter of iNOS gene [Int. Immunopharmacol. 3 (2003) 1353]. In the present study, we investigated the role of p38 kinase pathway and membrane receptors (CD14, Toll-like receptor 4 (TLR4), and CR3) in mediating nitric oxide (NO) production and NF-κB/Rel activation induced by PCSC. Treament of RAW 264.7 cells with PCSC resulted in significant activation of p38. The specific p38 inhibitor SB203580 abrogated the PCSC-induced NF-κB/Rel activation and NO generation, whereas the selective mitogen-activated protein kinase/extracellular signal-regulated kinase 1 (MEK-1) inhibitor PD98059 did not affect the NF-κB/Rel and NO induction. Treatment of RAW 264.7 cells with anti-CD14 Ab, anti-TLR4 Ab, and anti-CR3 Absignificantly blocked PCSC-induced NO production activation. In conclusion, we demonstrate that PCSC induces NF-κB/Rel activation and iNOS expression through the CD14, TLR4, and CR3 membrane receptor and p38 kinase which is critically involved in the signal transduction leading to NF-κB/Rel activation in murine macrophages.-
dc.publisherElsevier-
dc.titlePolysaccharide isolated from Poria cocos sclerotium induces NF-κB/Rel activation and iNOS expression through the activation of p38 kinase in murine macrophages-
dc.title.alternativePolysaccharide isolated from Poria cocos sclerotium induces NF-κB/Rel activation and iNOS expression through the activation of p38 kinase in murine macrophages-
dc.typeArticle-
dc.citation.titleInternational Immunopharmacology-
dc.citation.number8-
dc.citation.endPage1038-
dc.citation.startPage1029-
dc.citation.volume4-
dc.contributor.affiliatedAuthorJong Soon Kang-
dc.contributor.alternativeName이근영-
dc.contributor.alternativeName유호진-
dc.contributor.alternativeName정혜광-
dc.contributor.alternativeName강종순-
dc.contributor.alternativeName김환묵-
dc.contributor.alternativeName이상달-
dc.contributor.alternativeName전영진-
dc.identifier.bibliographicCitationInternational Immunopharmacology, vol. 4, no. 8, pp. 1029-1038-
dc.identifier.doi10.1016/j.intimp.2004.03.014-
dc.subject.keywordiNOS-
dc.subject.keywordMacrophages-
dc.subject.keywordNF-κB/Rel-
dc.subject.keywordp38 kinase-
dc.subject.keywordPoria cocos-
dc.subject.localiNOS-
dc.subject.localMacrophages-
dc.subject.localMacrophage-
dc.subject.localNF-κB/Rel-
dc.subject.localp38 kinase-
dc.subject.localPoria cocos-
dc.description.journalClassY-
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Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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