Layered structure of granules in upflow anaerobic sludge blanket reactor gives microbial populations resistance to metal ions

Abstract

Metal ions (Cd2+, Cu2+, Ni2+, Zn2+ and Cr3+) did not affect glucose degradation or the production of methane during anaerobic digestion with intact and disintegrated granules from a UASB (Upflow Anaerobic Sludge Blanket) reactor. However, when Cu2+ was at 500 mg g-1 VSS (volatile suspended solids) in the media, the glucose degradation rates and methane production rates decreased by 14% and 32% in disintegrated granules, respectively, whereas, in intact granules, decreases were 3% and 14%, respectively. When various electroplating metal ions were tested, 50% inhibition of acetate degradation and methane production were produced by 210-770 mg g-1 VSS and 120-630 mg g-1 VSS, respectively. The relative toxicity of the electroplating metals on methane production was in the order of Zn2+ (most toxic) > Ni2+ > Cu2+ > Cr3+ > Cd2+ (least toxic).