High production of L-threonine using controlled feeding of L-methionine and phosphate by Escherichia coli mutant = L-methionine과 phosphate의 제한 공급에 의한 Escherichia coli MT201로부터의 고농도 L-threonine 생산

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dc.contributor.authorMan Hyo Lee-
dc.contributor.authorHong-Weon Lee-
dc.contributor.authorB J Kim-
dc.contributor.authorChun Suk Kim-
dc.contributor.authorJoon Ki Jung-
dc.contributor.authorY I Hwang-
dc.date.accessioned2017-04-19T09:01:16Z-
dc.date.available2017-04-19T09:01:16Z-
dc.date.issued2004-
dc.identifier.issn0257-2389-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6564-
dc.description.abstractL-Threonine fermentation process was constructed on batch and fed-batch culture by using Escherichia coli MT201. The production type of L-threonine was observed as growth-associated production in batch culture. In fed-batch culture studying optimal concentration of yeast extract in feeding media, when 600 g/l of glucose and 60 g/l of yeast extract were added in feeding media, 87 g/l of L-threonine was produced. To improve cell growth and L-threonine production, the culture of high cell density was performed in fed-batch culture with oxygen enriched air and feeding media containing L-methionine and phosphate. Under the conditions, we could achieve the highest L-threonine production of 98 g/l at 60 h. The highest productivity of L-threonine was about 3.85 g/l/h.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleHigh production of L-threonine using controlled feeding of L-methionine and phosphate by Escherichia coli mutant = L-methionine과 phosphate의 제한 공급에 의한 Escherichia coli MT201로부터의 고농도 L-threonine 생산-
dc.title.alternativeHigh production of L-threonine using controlled feeding of L-methionine and phosphate by Escherichia coli mutant-
dc.typeArticle-
dc.citation.titleKorean Journal of (Applied) Microbiology & Biotechnology-
dc.citation.number2-
dc.citation.endPage153-
dc.citation.startPage149-
dc.citation.volume32-
dc.contributor.affiliatedAuthorMan Hyo Lee-
dc.contributor.affiliatedAuthorHong-Weon Lee-
dc.contributor.affiliatedAuthorChun Suk Kim-
dc.contributor.affiliatedAuthorJoon Ki Jung-
dc.contributor.alternativeName이만효-
dc.contributor.alternativeName이홍원-
dc.contributor.alternativeName김병진-
dc.contributor.alternativeName김천석-
dc.contributor.alternativeName정준기-
dc.contributor.alternativeName황용일-
dc.identifier.bibliographicCitationKorean Journal of (Applied) Microbiology & Biotechnology, vol. 32, no. 2, pp. 149-153-
dc.subject.keywordescherichia coli MT201-
dc.subject.keywordL-Methionine-
dc.subject.keywordL-Threonine production-
dc.subject.keywordphosphate-
dc.subject.keywordyeast extract-
dc.subject.localEscherichia coli MT201-
dc.subject.localescherichia coli MT201-
dc.subject.localL-Methionine-
dc.subject.locall-methionine-
dc.subject.localL-Threonine production-
dc.subject.localPhosphate-
dc.subject.localphosphate-
dc.subject.localyeast extract-
dc.description.journalClassN-
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Division of Bio Technology Innovation > 1. Journal Articles
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