Impeller types and feeding modes influence the morphology and protein expression in the submerged culture of Aspergillus oryzae

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dc.contributor.authorJoo-Hyung Heo-
dc.contributor.authorV Ananin-
dc.contributor.authorJeong Seok Park-
dc.contributor.authorChung Ryul Lee-
dc.contributor.authorJ O Moon-
dc.contributor.authorOh Suk Kwon-
dc.contributor.authorHyun Ah Kang-
dc.contributor.authorChul Ho Kim-
dc.contributor.authorSang Ki Rhee-
dc.date.accessioned2017-04-19T09:01:17Z-
dc.date.available2017-04-19T09:01:17Z-
dc.date.issued2004-
dc.identifier.issn1226-8372-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6566-
dc.description.abstractThe influences of impeller types on morphology and protein expression were investigated in a submerged culture of Aspergillus oryzae. The impeller types strongly affected mycelial morphology and protein production in batch and fed-batch fermentations. Cells that were cultured by propeller agitation grew in the form of a pellet, whereas cells that were cultured by turbine agitation grew in a freely dispersed-hyphal manner and in a clumped form. Pellet-grown cells showed high levels of protein production for both the intracellularly heterologous protein (β-glucuronidase) and the extracellularly homologous protein (α-amylase). The feeding mode of the carbon source also influenced the morphological distribution and protein expression in fed-batch fermentation of A. oryzae. Pulsed-feeding mainly showed high protein expression and homogeneous distribution of pellet whereas continuous feeding resulted in less protein expression and heterogeneous distribution with pellet and dispersed-hyphae. The pellet growth with propeller agitation paralleling with the pulsed-feeding of carbon source showed a high level of protein production in the submerged fed-batch fermentation of recombinant A. oryzae.-
dc.publisherSpringer-
dc.titleImpeller types and feeding modes influence the morphology and protein expression in the submerged culture of Aspergillus oryzae-
dc.title.alternativeImpeller types and feeding modes influence the morphology and protein expression in the submerged culture of Aspergillus oryzae-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number3-
dc.citation.endPage190-
dc.citation.startPage184-
dc.citation.volume9-
dc.contributor.affiliatedAuthorJoo-Hyung Heo-
dc.contributor.affiliatedAuthorJeong Seok Park-
dc.contributor.affiliatedAuthorChung Ryul Lee-
dc.contributor.affiliatedAuthorOh Suk Kwon-
dc.contributor.affiliatedAuthorHyun Ah Kang-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.alternativeName허주형-
dc.contributor.alternativeNameAnanin-
dc.contributor.alternativeName박정석-
dc.contributor.alternativeName이충열-
dc.contributor.alternativeName문준옥-
dc.contributor.alternativeName권오석-
dc.contributor.alternativeName강현아-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName이상기-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 9, no. 3, pp. 184-190-
dc.identifier.doi10.1007/BF02942290-
dc.subject.keywordaspergillus oryzae-
dc.subject.keywordfed-batch fermentation-
dc.subject.keywordfilamentous fungi-
dc.subject.keywordmorphology-
dc.subject.keywordpropeller agitation-
dc.subject.keywordsubmerged culture-
dc.subject.localaspergillus oryzae-
dc.subject.localFed-batch fermentation-
dc.subject.localfed-batch fermentation-
dc.subject.localfilamentous fungi-
dc.subject.localmorphology-
dc.subject.localMorphology-
dc.subject.localpropeller agitation-
dc.subject.localsubmerged culture-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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