Development of a competitive direct enzyme-linked immunosorbent assay for teicoplanin

Cited 0 time in scopus
Metadata Downloads
Title
Development of a competitive direct enzyme-linked immunosorbent assay for teicoplanin
Author(s)
H B Lee; B Y Kwak; Jae-Chan Lee; Chang-Jin Kim; D H Shon
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 14, no. 3, pp. 612-619
Publication Year
2004
Abstract
A competitive direct enzyme-linked immunosorbent assay (cdELISA) was developed for selective and rapid detection of a glycopeptide antibiotic, teicoplanin (TP). TP was conjugated to bovine serum albumin (BSA) for use as an immunogen. Repeated subcutaneous injections of 0.5 mg of the conjugate was effective in generating specific polyclonal antibody (PAb) toward TP in rabbits, as determined by cdELISA. TP-horseradish peroxidase conjugate (TP-HRP) was used as an enzyme marker. The cdELISA was developed based on a competition reaction between TP-BSA PAb and TP-HRP conjugate. The TP-BSA PAb was highly sensitive (detection limit, 0.3 ng/ml) and specific toward teicoplanin, showing no cross-reactivity to other glycopeptide antibiotics including vancomycin. There were good correlations (r2=0.84 and 0.76, respectively) between cdELISA and microbiological assay, and high-performance liquid chromatography. The cdELISA system developed in this work is expected to be useful not only for selective and rapid monitoring of TP but also for study of TP pharmacokinetics.
Keyword
competitive enzyme-linked immunosorbent assaypolyclonal antibodyteicoplaninactinoplanes teichomyceticus
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.