Expression of a human histone H1.5 in transgenic tobacco cultured cells = 담배 배양세포에서 인간 히스톤 단백질 H1.5의 발현

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Title
Expression of a human histone H1.5 in transgenic tobacco cultured cells = 담배 배양세포에서 인간 히스톤 단백질 H1.5의 발현
Author(s)
K Y Kim; Suk Yoon Kwon; J Y Song; Haeng Soon Lee; Sang Soo Kwak
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 31, no. 2, pp. 175-178
Publication Year
2004
Abstract
Transgenic tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cell lines expressing a human histone H1.5 (referred to as hH1.5), which suppress collagen-induced rheumatoid arthritis, were developed under the oxidative stress-inducible peroxidase (SWPA2) promoter. Tobacco BY-2 cells were transformed by Agrobacterium-mediated method. The kanamycin-resistant calli were selected on the modified MS medium containing 150mg/L kanamycin and 300mg/L claforan. Transgenic cell lines were confirmed by PCR and northern blot analysis. Recombinant hH1.5 (rhH1.5) protein (42 kDa) was also detected by Western blot analysis, showing a different molecular weight of human hH1.5 (32 kDa). These results suggested that a hH1.5 gene was properly introduced in tobacco cultured cells under the control of SWPA2 promoter. The further characterization of rhH1.5 protein remains to be studied.
Keyword
collagen-induced rheumatoid arthritiscultured cellshistone H1.5oxidative stress-inducible SWPA2 promoter
ISSN
1015-5880
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2004.31.2.175
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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