Selection of transgenic potato plants expressing both CuZnSOD and APX in chloroplasts with enhanced tolerance to oxidative stress = CuZnSOD와 APX를 엽록체에 발현시킨 산화스트레스 내성 형질전환 감자의 선발

Cited 0 time in scopus
Metadata Downloads
Title
Selection of transgenic potato plants expressing both CuZnSOD and APX in chloroplasts with enhanced tolerance to oxidative stress = CuZnSOD와 APX를 엽록체에 발현시킨 산화스트레스 내성 형질전환 감자의 선발
Author(s)
Li Tang; Suk Yoon KwonSang Soo Kwak; C K Sung; Haeng Soon Lee
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 31, no. 2, pp. 109-113
Publication Year
2004
Abstract
n order to develop transgenic potato plants with enhanced tolerance to multiple stress, we constructed the transformation vector expressing both superoxide dismutase and ascorbate peroxidase genes in chloroplasts under the control of a stress-inducible SWPA2 promoter. Transgenic potato plants (cv. Superior and Atlantic) were generated using an Agrobacterium-mediated transformation system. Transgenic potato plants were regenerated on MS medium containing 100mg/L kanamycin. Genomic Southern blot analysis confirmed the incorporation of foreign genes into the potato genome. When potato leaf discs were subjected to methyl viologen (MV) at 10 \muM, transgenic plants showed higher tolerance than non-transgenic or vector-transformed plants. To further study we selected the transgenic plant lines with enhanced tolerance against MV. These plants will be used for further analysis of stress-tolerance to multiple environmental stresses.
Keyword
ascorbate peroxidaseoxidative stresssolanum tubersumsuperoxidedismutasetransformation
ISSN
1015-5880
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2004.31.2.109
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.