Immortalization of rabbit corneal fibroblasts by overexpression of simian virus 40 large T antigen

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dc.contributor.authorSeung Ju Cho-
dc.contributor.authorYuk-Pheel Park-
dc.contributor.authorH M Lim-
dc.contributor.authorJ C Kim-
dc.contributor.authorE K Yang-
dc.contributor.authorJ K Park-
dc.contributor.authorDo Young Yoon-
dc.contributor.authorHee Gu Lee-
dc.date.accessioned2017-04-19T09:01:18Z-
dc.date.available2017-04-19T09:01:18Z-
dc.date.issued2004-
dc.identifier.issnI000-0011-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6575-
dc.description.abstractImmortalization of primary corneal cells has influence on pharmacy, medical and biological fields. Especially, investigation of immortalization mechanism using viral oncoproteins is useful for medical treatments, and these cell lines will be useful materials for toxic test of medical supplies and cell biological experiments. Rabbit corneal fibroblasts in culture undergo a finite number of divisions before they reach a terminally non-proliferating state known as replicative senescence. Therefore, we attempted to induce immortalization of rabbit corneal fibroblasts with SV40 large T antigen. As a result of experiment, expression of SV40 large T antigen was confirmed, and expression of proteins related to cell cycle repressor was decreased in the transfection group compared with non-transfection group. According to the results of cell cycle phase distribution test, SV40 large T antigen-transfected cells had obtained higher proliferation rate than primary cells. It was confirmed that during induction of immortalization, SV40 large T antigen was not able to increase telomerase activity. In conclusion, we made a rabbit corneal fibroblast cell line with SV40 large T antigen. This cell line will be useful for further studies of mammalian fibroblast biology, particularly with regard to angiogenesis and malignant transformation. In addition, this cell line offers opportunity for testing potential therapeutics and can be used for toxicity tests of materials or cosmetics. In the future, our cell line can potentially be utilized in a wide range of biology related fields.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleImmortalization of rabbit corneal fibroblasts by overexpression of simian virus 40 large T antigen-
dc.title.alternativeImmortalization of rabbit corneal fibroblasts by overexpression of simian virus 40 large T antigen-
dc.typeArticle-
dc.citation.titleKorean Journal of Experimental & Biomedical Science-
dc.citation.number2-
dc.citation.endPage92-
dc.citation.startPage85-
dc.citation.volume10-
dc.contributor.affiliatedAuthorSeung Ju Cho-
dc.contributor.affiliatedAuthorYuk-Pheel Park-
dc.contributor.affiliatedAuthorDo Young Yoon-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName조승주-
dc.contributor.alternativeName박육필-
dc.contributor.alternativeName임헌만-
dc.contributor.alternativeName김재찬-
dc.contributor.alternativeName양은경-
dc.contributor.alternativeName박종극-
dc.contributor.alternativeName윤도영-
dc.contributor.alternativeName이희구-
dc.identifier.bibliographicCitationKorean Journal of Experimental & Biomedical Science, vol. 10, no. 2, pp. 85-92-
dc.subject.keywordimmortalization-
dc.subject.keywordSV40 large T antigen-
dc.subject.keywordtelomerase-
dc.subject.localimmortalization-
dc.subject.localImmortalization-
dc.subject.localSV40 large T antigen-
dc.subject.localtelomerase-
dc.subject.localTelomerase-
dc.description.journalClassN-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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