Lactoferrin works as a new LPS-binding protein in inflammatory activation of macrophages

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Title
Lactoferrin works as a new LPS-binding protein in inflammatory activation of macrophages
Author(s)
Y J Na; Sang Bae Han; Jong Soon Kang; Yeo Dae Yoon; Song Kyu Park; Hwan Mook Kim; Kyu-Hwan Yang; C O Joe
Bibliographic Citation
International Immunopharmacology, vol. 4, no. 9, pp. 1187-1199
Publication Year
2004
Abstract
Though lactoferrin (LF) is a glycoprotein that is involved in immunomodulation, its action mechanism has not been fully elucidated. Previous studies have suggested that lipopolysaccharide (LPS) activity is inhibited by direct binding between LPS and LF. However, here we show that when LPS and purified LF was mixed, and formed a complex (termed as LF-LPS), it was found to induce production of inflammatory mediators in macrophages to some extent, rather than inhibit LPS activity. Moreover, when macrophages were pretreated with LF-LPS, cells were rendered a tolerant state to LPS challenge. These macrophage-activating effects were mediated by Toll-like receptor 4 (TLR4)-NF-κB pathway. Comparative studies with C3H/HeN and C3H/HeJ mice demonstrated the strong dependency of the LF-LPS signal on TLR4. These findings suggest that the immunomodulatory properties of LF could be due, in part, to LPS binding.
Keyword
LactoferrinLipopolysaccharideMacrophageToll-like receptor 4
ISSN
1567-5769
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.intimp.2004.05.009
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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