Cloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4

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dc.contributor.authorNack Shick Choi-
dc.contributor.authorKyu Tae Chang-
dc.contributor.authorP J Maeng-
dc.contributor.authorSeung Ho Kim-
dc.date.accessioned2017-04-19T09:01:19Z-
dc.date.available2017-04-19T09:01:19Z-
dc.date.issued2004-
dc.identifier.issn0378-1097-
dc.identifier.uri10.1016/j.femsle.2004.06.006ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6583-
dc.description.abstractPreviously, we purified a strong fibrinolytic enzyme (subtilisin DJ-4) from Bacillus sp. DJ-4 and characterized its enzymatic activity. Here, we cloned the gene subtilisin DJ-4, and determines its nucleotide sequence, which showed 97% identity with subtilisin BPN′ from B. amyloliquefacens. Recombinant full-subtilisin DJ-4 (rf-subDJ-4) and mature-subtilisin DJ-4 (rm-subDJ-4) were expressed using a pET29 vector system, and their fibrin (ogen)olytic and plasminogen activator activities were studied. rf-subDJ-4 was found to have a higher stability to heat (60°C) and to acidic conditions (pH 3.0-4.0) than the native subtilisin DJ-4 of Bacillus sp. DJ-4. The plasminogen activator activity of rf-subDJ-4 was 2.75 times greater than that of plasmin on a molar basis. And its specific activity (F/C, the ratio of fibrinolytic activity to caseinolytic activity) was 2.67 and 3.97 times higher than those of subtilisin BPN′ and subtilisin Carlsberg, respectively. rf-subDJ-4 rapidly hydrolyzed the Aα-, Bβ-, and γ-chains of fibrinogen within 5 min. But, unlike subtilisin BPN′ at a very low concentration (50 ng), the γ-chain was not cleaved. On the other hand, rm-subDJ-4 did not show enzyme activity.-
dc.publisherOxford Univ Press-
dc.titleCloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4-
dc.title.alternativeCloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4-
dc.typeArticle-
dc.citation.titleFEMS Microbiology Letters-
dc.citation.number2-
dc.citation.endPage331-
dc.citation.startPage325-
dc.citation.volume236-
dc.contributor.affiliatedAuthorNack Shick Choi-
dc.contributor.affiliatedAuthorKyu Tae Chang-
dc.contributor.affiliatedAuthorSeung Ho Kim-
dc.contributor.alternativeName최낙식-
dc.contributor.alternativeName장규태-
dc.contributor.alternativeName맹필재-
dc.contributor.alternativeName김승호-
dc.identifier.bibliographicCitationFEMS Microbiology Letters, vol. 236, no. 2, pp. 325-331-
dc.identifier.doi10.1016/j.femsle.2004.06.006-
dc.subject.keywordBacillus-
dc.subject.keywordFibrinolytic enzyme-
dc.subject.keywordPlasminogen activator-
dc.subject.keywordRecombinant subtilisin DJ-4-
dc.subject.localBacillus-
dc.subject.localbacillus-
dc.subject.localFibrinolytic enzyme-
dc.subject.localFibrinolytic enzymes-
dc.subject.localfibrinolytic enzyme-
dc.subject.localPlasminogen activator-
dc.subject.localRecombinant subtilisin DJ-4-
dc.description.journalClassY-
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