Transgenic plants of easter lily(Lilium longiflorum) with phosphinothricin resistance

Abstract

Transient uidA expression was used to optimize parameters required for biolistic transformation of suspension cells of Easter lily, Lilium longiflourm. Maximum uidA expression occurred following bombardment with gold particles as compared to tungsten. A 3 hr pre-treatment of suspension cells with 0.125 M osmoticum resulted in a 1.5X increase in uidA expression. A helium pressure of 1550 psi combined with a particle travelling distance of 6 cm resulted in maximum uidA expression as compared to either 1100, 1200, or 1800 psi.