Genetic monitoring using biochemical and microsatellite marker of KCR rat = 생화학적 마커와 microsatellite 마커를 이용한 KCR 랫드의 유전모니터링

Abstract

The cataract mutant rat which showed lens opacity was found in the colony of Sprague-Dawley rat at Korea Research Institute of Chemical Technology (KRICT) in 1996. The mode of inheritance of affected rats was autosomal recessive. These cataractous rats were mated by sister and brother to 27th (F27) generation and named as Korea Cataract Rat (KCR). We performed genetic monitoring of KCR rat using biochemical and microsatellite markers to determine their genetic identified homozygous and distinctive genetic profile. In biochemical monitoring, six rats from each of the F18/F19/F20 were tested using 13 biochemical markers. In molecular genetic monitoring, nine rats from the F25 and F26 were tested using 13 microsatellite markers. No differences were found among the genetic profiles of the F18/F19/F20 in biochemical monitoring. Also, in the molecular genetic monitoring, we identified that both genetic profiles of F25 and F26 were the same. The genetic profile of KCR rats was unique against the other major rat strains. In addition, the polymorphysm of KCR rat for microsatellite markers was different from the other strains. These results suggest that KCR rat is genetically well qualified as far as in this test and it has been well established as a new inbred strain which has a novel genetic profile and cataract trait.