Athb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion

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Title
Athb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion
Author(s)
D J Shin; Y D Koo; Jiyoung Lee; H J Lee; D W Baek; S C Lee; C I Cheon; Sang Soo Kwak; S Y Lee; D J Yun
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 323, no. 2, pp. 534-540
Publication Year
2004
Abstract
An Arabidopsis cDNA clone that encodes Athb-12, a homeobox-leucine zipper domain protein (HD-Zip), was isolated by functional complementation of the NaCl-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant (cnbΔ, regulatory subunit null). CaN, a Ca2+/calmodulin- dependent protein phosphatase, regulates Na+ ion homeostasis in yeast. Expression of Athb-12 increased NaCl tolerance but not osmotic stress tolerance of these cnbΔ cells. Furthermore, expression of two other HD-Zip from Arabidopsis, Athb-1 and -7, did not suppress NaCl sensitivity of cnbΔ cells. These results suggest that Athb-12 specifically functions in Na+ ion homeostasis in yeast. Consistent with these observations, expression of Athb-12 in yeast turned on transcription of the NaCl stress-inducible PMR2A, which encodes a Na+/Li+ translocating P-type ATPase, and decreased Na+ levels in yeast cells. To investigate the biological function of Athb-12 in Arabidopsis, we performed Northern blot analysis. Expression of Athb-12 was dramatically induced by NaCl and ABA treatments, but not by KCl. In vivo targeting experiments using a green fluorescent protein reporter indicated that Athb-12 was localized to the nucleus. These results suggest that Athb-12 is a putative transcription factor that may be involved in NaCl stress responses in plants.
Keyword
A homeobox-leucine zipper domain proteinArabidopsisNa + ion homeostasisSalt toleranceYeastzipper protein
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2004.08.127
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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