Athb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion

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dc.contributor.authorD J Shin-
dc.contributor.authorY D Koo-
dc.contributor.authorJiyoung Lee-
dc.contributor.authorH J Lee-
dc.contributor.authorD W Baek-
dc.contributor.authorS C Lee-
dc.contributor.authorC I Cheon-
dc.contributor.authorSang Soo Kwak-
dc.contributor.authorS Y Lee-
dc.contributor.authorD J Yun-
dc.date.accessioned2017-04-19T09:01:25Z-
dc.date.available2017-04-19T09:01:25Z-
dc.date.issued2004-
dc.identifier.issn0006291X-
dc.identifier.uri10.1016/j.bbrc.2004.08.127ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6609-
dc.description.abstractAn Arabidopsis cDNA clone that encodes Athb-12, a homeobox-leucine zipper domain protein (HD-Zip), was isolated by functional complementation of the NaCl-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant (cnbΔ, regulatory subunit null). CaN, a Ca2+/calmodulin- dependent protein phosphatase, regulates Na+ ion homeostasis in yeast. Expression of Athb-12 increased NaCl tolerance but not osmotic stress tolerance of these cnbΔ cells. Furthermore, expression of two other HD-Zip from Arabidopsis, Athb-1 and -7, did not suppress NaCl sensitivity of cnbΔ cells. These results suggest that Athb-12 specifically functions in Na+ ion homeostasis in yeast. Consistent with these observations, expression of Athb-12 in yeast turned on transcription of the NaCl stress-inducible PMR2A, which encodes a Na+/Li+ translocating P-type ATPase, and decreased Na+ levels in yeast cells. To investigate the biological function of Athb-12 in Arabidopsis, we performed Northern blot analysis. Expression of Athb-12 was dramatically induced by NaCl and ABA treatments, but not by KCl. In vivo targeting experiments using a green fluorescent protein reporter indicated that Athb-12 was localized to the nucleus. These results suggest that Athb-12 is a putative transcription factor that may be involved in NaCl stress responses in plants.-
dc.publisherElsevier-
dc.titleAthb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion-
dc.title.alternativeAthb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number2-
dc.citation.endPage540-
dc.citation.startPage534-
dc.citation.volume323-
dc.contributor.affiliatedAuthorJiyoung Lee-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.alternativeName신동진-
dc.contributor.alternativeName구윤덕-
dc.contributor.alternativeName이지영-
dc.contributor.alternativeName이효정-
dc.contributor.alternativeName백동원-
dc.contributor.alternativeName이석찬-
dc.contributor.alternativeName천충일-
dc.contributor.alternativeName곽상수-
dc.contributor.alternativeName이상열-
dc.contributor.alternativeName윤대진-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 323, no. 2, pp. 534-540-
dc.identifier.doi10.1016/j.bbrc.2004.08.127-
dc.subject.keywordA homeobox-leucine zipper domain protein-
dc.subject.keywordArabidopsis-
dc.subject.keywordNa + ion homeostasis-
dc.subject.keywordSalt tolerance-
dc.subject.keywordYeastzipper protein-
dc.subject.localA homeobox-leucine zipper domain protein-
dc.subject.localArabidopsis-
dc.subject.localNa + ion homeostasis-
dc.subject.localSalt tolerance-
dc.subject.localYeastzipper protein-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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