A novel design of simulated moving bed(SMB) chromatography for separation of ketoprofen enantiomer

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Title
A novel design of simulated moving bed(SMB) chromatography for separation of ketoprofen enantiomer
Author(s)
T H Yoon; Bong Hyun Chung; I H Kim
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 9, no. 4, pp. 285-291
Publication Year
2004
Abstract
A simulated moving bed (SMB) chromatography system is a powerful tool for preparative scale separation, which can be applied to the separation of chiral compound. We have designed our own lab-scale SMB chromatography using 5 HPLC pumps, 6 stainless steel columns and 4 multi-position valves, to separate a racemic mixture of ketoprofen in to its enantiomers. Our design has the characteristics of the low cost for assembly for the SMB chromatography and easy repair of the unit, which differs from the designs suggested by other investigators. It is pos-sible for the flow path through each column to be independently changed by computer control, using 4 multi-position rotary valves and 5 HPLC solvent delivery pumps. In order to prove the operability of our SMB system, attempts were made to separate the (S)-ketoprofen enantiomer from a ketoprofen racemic mixture. The operating parameters of the SMB chromatography were calculated for ketoprofen separation from a batch chromatography experiment as well as by the triangle theory. With a feed concentration of 1 mg/mL, (S)-ketoprofen was obtained with a purity of 96% under the calculated operating conditions.
Keyword
SMB chromatographychiral separationketoprofen enantiomerrotary valve
ISSN
1226-8372
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF02942345
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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