Adeno-associated virus Rep78 binds to E2-responsive element 1 of bovine papillomavirus type 1

Cited 2 time in scopus
Metadata Downloads
Title
Adeno-associated virus Rep78 binds to E2-responsive element 1 of bovine papillomavirus type 1
Author(s)
S K Chon; B M Rim; Dong Soo Im
Bibliographic Citation
IUBMB Life, vol. 48, no. 4, pp. 397-404
Publication Year
1999
Abstract
Adeno-associated virus type-2 (AAV-2) is a helper-dependent parvovirus that has been implicated in the inhibition of replication and oncogenic transformation of bovine papillomavirus type-1 (BPV-1) and other transforming DNA viruses. Previous studies have suggested that the Rep78 protein of AAV-2 is a key player mediating this effect. In this report we have analyzed the effect of AAV-2 Rep78 protein on the regulation of gene expression of a reporter gene under the control of the long control region (LCR) of BPV-1. Our results show that Rep78 is capable of down-regulating the promoter activity of the LCR in vivo in tissue culture cells. Inhibition of LCR activity in vivo suggested the need for Rep78 to bind to a region of the LCR promoter spanning the E2-responsive elements of BPV-1. This observation was further confirmed in vitro with gel shift assays showing specific binding of Rep78 to DNA oligonucleotides containing E2-responsive element 1 (E2RE1) sequences of BPV-1 LCR. Our results expand the understanding of the mechanism of trans-regulation mediated by Rep78 and involving this protein and DNA sequences with complex secondary structure.
Keyword
Adeno-associated virus type-2bovine papillomavirus type-1E2-responsive element 1Rep78.
ISSN
1521-6543
Publisher
Wiley
DOI
http://dx.doi.org/10.1080/713803543
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.