Analysis of microbial communities during cyanobacterial bloom in Daechung reservoir by DGGE = DGGE를 이용한 대청호 수화 발생시기의 세균군집 분석

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Title
Analysis of microbial communities during cyanobacterial bloom in Daechung reservoir by DGGE = DGGE를 이용한 대청호 수화 발생시기의 세균군집 분석
Author(s)
So-Ra Ko; S J Park; Chi-Yong Ahn; Ae-Ran Choi; Jung-Sook LeeHee Sik Kim; Byung Dae Yoon; Hee-Mock Oh
Bibliographic Citation
Korean Journal of Microbiology, vol. 40, no. 3, pp. 205-210
Publication Year
2004
Abstract
The change of bacterial communities during cyanobacterial bloom was analyzed by DGGE in Daechung Reservoir from July to October in 2003. The traditional morphological analysis showed that the genera of Microcystis, Chroococcus, Oscillatoria, and Phormidium were dominated. The most frequent band in the DGGE profile by 16S rDNA sequence analysis was identified as Microcystis flos-aquae and the cyanobacterial bloom was peaked on September 2. Oscillatoria spp. were also identified and Aphanizomenon flos-aquae dominated in the middle of August. Judging from the analysis of the digitalized DGGE profiles using the cluster analysis technique, the microbial community on September 2 was considerably different from others. Consequently, it seems that the gene fingerprinting method can give not only the similar results to the traditional morphological method but also additional information on the bacterial species and similarity among the examined microbial communities.
Keyword
algal bloomcyanobacteriaDGGE (denaturing gradient gel electrophoresis)16S rDNA
ISSN
0440-2413
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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