Quantitative Determination of Bitter Principles in Specimens of Ganoderma lucidum Using High-Performance Liquid Chromatography and its application to the evaluation of Ganoderma products

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Title
Quantitative Determination of Bitter Principles in Specimens of Ganoderma lucidum Using High-Performance Liquid Chromatography and its application to the evaluation of Ganoderma products
Author(s)
J J Gao; N Nakamura; Byung Sun Min; A Hirakawa; F Zuo; M Hattori
Bibliographic Citation
Chemical & Pharmaceutical Bulletin, vol. 52, no. 6, pp. 688-695
Publication Year
2004
Abstract
6) and 13 ganoderma acids (7-19), in the products of Ganoderma lucidum, an analytical system was developed using high-performance liquid chromatography with an ODS column. The mobile phase was a linear gradient of 1% AcOH/H2O-CH3CN and 2% AcOH/H2O-CH 3CN, and the elution profile was monitored at 243 and 250 nm for ganoderma alcohols and acids, respectively. The relative standard deviations of this method were less than 2.35% and 2.18% (n=5) for intraday and interday assays, and the recoveries were 90.9-100.8% and 93.4-103.9% for constituents of alcohol and acid groups, respectively. This system was applied to a quantitative determination of the constituents in 10 different products of G. lucidum: six usual umbrella forms of the fruiting bodies, three antlered forms of the fruiting bodies and spores, and eight specimens from the same G. lucidum strain, which was parasitized on logs from different plants or different fungus beds. The analytical results indicated that the quantity and composition of these triterpenes differed appreciably among various specimens, but the relative ratio of the alcohols and acids was not significantly different when the same strain of G. lucidum was used.
Keyword
antlered formfruiting bodyganoderma lucidumHPLCsporetriterpene
ISSN
0009-2363
Publisher
Pharmaceutical Soc Japan
DOI
http://dx.doi.org/10.1248/cpb.52.688
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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