Mutational analysis of Xanthomonas harpin HpaG identifies a key functional region that elicits the hypersensitive response in nonhost plants

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dc.contributor.authorJ G Kim-
dc.contributor.authorE K Jeon-
dc.contributor.authorJ H Oh-
dc.contributor.authorMoon Jae Sun-
dc.contributor.authorI G Hwang-
dc.date.accessioned2017-04-19T09:01:40Z-
dc.date.available2017-04-19T09:01:40Z-
dc.date.issued2004-
dc.identifier.issn00219193-
dc.identifier.uri10.1128/JB.186.18.6239-6247.2004ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6661-
dc.description.abstractHpaG is a type III-secreted elicitor protein of Xanthomonas axonopodis pv. glycines. We have determined the critical amino acid residues important for hypersensitive response (HR) elicitation by random and site-directed mutagenesis of HpaG and its homolog XopA. A plasmid clone carrying hpaG was mutagenized by site-directed mutagenesis, hydroxylamine mutagenesis, and error-prone PCR. A total of 52 mutants were obtained, including 51 single missense mutants and 1 double missense mutant. The HR elicitation activity was abolished in the two missense mutants [HpaG(L50P) and HpaG(L43P/L50P)]. Seven single missense mutants showed reduced activity, and the HR elicitation activity of the rest of the mutants was similar to that of wild-type HpaG. Mutational and deletion analyses narrowed the region essential for elicitor activity to the 23-amino-acid peptide (H2N-NQGISEKQLDQLLTQLIMALLQQ-COOH). A synthetic peptide of this sequence possessed HR elicitor activity at the same concentration as the HpaG protein. This region has 78 and 74% homology with 23- and 27-amino-acid regions of the HrpW harpin domains, respectively, from Pseudomonas and Erwinia spp. The secondary structure of the peptide is predicted to be an α-helix, as is the HrpW region that is homologous to HpaG. The predicted α-helix of HpaG is probably critical for the elicitation of the HR in tobacco plants. In addition, mutagenesis of a xopA gene yielded two gain-of-function mutants: XopA(F48L) and XopA(F48L/M52L). These results indicate that the 12 amino acid residues between L39 and L50 of HpaG have critical roles in HR elicitation in tobacco plants.-
dc.publisherAmer Soc Microb-
dc.titleMutational analysis of Xanthomonas harpin HpaG identifies a key functional region that elicits the hypersensitive response in nonhost plants-
dc.title.alternativeMutational analysis of Xanthomonas harpin HpaG identifies a key functional region that elicits the hypersensitive response in nonhost plants-
dc.typeArticle-
dc.citation.titleJournal of Bacteriology-
dc.citation.number18-
dc.citation.endPage6247-
dc.citation.startPage6239-
dc.citation.volume186-
dc.contributor.affiliatedAuthorMoon Jae Sun-
dc.contributor.alternativeName김정건-
dc.contributor.alternativeName전은경-
dc.contributor.alternativeName오종희-
dc.contributor.alternativeName문제선-
dc.contributor.alternativeName황인규-
dc.identifier.bibliographicCitationJournal of Bacteriology, vol. 186, no. 18, pp. 6239-6247-
dc.identifier.doi10.1128/JB.186.18.6239-6247.2004-
dc.description.journalClassY-
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Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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