Phenylpropanoids from Myristica fragrans houtt = 육두구(Myristica fragrans Houtt)로부터 phenylpropanoid의 분리

Abstract

Myristica fragrans Houtt were extracted in 80% aq. MeOH and solvent fractionated using CHCl3, EtOAc, n-BuOH and water, successively. The n-BuOH fraction gave three phenylpropanoids through application of silica gel column chromatographies. The chemical structures of the phenylpropanoids were determined by the interpretation of several spectral data, including NMR and MS as meso-dihydroguaiaretic acid (1), nectandrin B (2) and syringin methyl ether (3). Compound 1, which was first isolated from this plant by authors, showed inhibitory activities with 60.0?2.1% (100 μg/ml), 42.6?0.9% (140 μg/ml) and 12.2?0.2% (200 μg/ml) on ACAT(acyl-CoA:Cholesterol Acyltransferase), chitin synthase III and HMG-CoA reductase (3-hydroxy-3-methylglutaryl coenzyme A reductase), respectively. Compound 3 showed inhibitory activities with 27.2?0.9% (100 μg/ml), 45.5?0.8% (200 μg/ml) on ACAT and chitin synthase III.