Pathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway

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Title
Pathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway
Author(s)
C J Park; K J Kim; R Shin; Jeong Mee Park; Y C Shin; K H Paek
Bibliographic Citation
Plant Journal, vol. 37, no. 2, pp. 186-198
Publication Year
2004
Abstract
A hot pepper (Capsicum annuum) cDNA clone encoding pathogenesis-related protein 10 (CaPR-10) was isolated by differential screening of a cDNA library prepared from pepper leaves inoculated with tobacco mosaic virus pathotype (TMV-P0). CaPR-10 transcripts were induced in the incompatible interaction with TMV-P0 or Xanthomonas campestris pv. vesicatoria (Xcv) but not induced in the compatible interaction. Characterization of enzymatic properties of CaPR-10 indicated that the recombinant protein exhibits a ribonucleolytic activity against TMV RNA, as well as against pepper total RNA, and shows its putative antiviral activity in several conditions. The CaPR-10 protein existed at very low level in leaf tissue but was dramatically induced as soon as plants were inoculated with TMV-P0, and this was correlated with the increase of its ribonucleolytic activity. Immunoblot analysis and pull-down assays using proteins extracted from pepper leaves showed that TMV-P0 inoculation led to the phosphorylation of CaPR-10, a modification that should affect its capacity for RNase function. We present data that the induction and subsequent phosphorylation of CaPR-10 increased its ribonucleolytic activity to cleave invading viral RNAs, and this activity should be important to its antiviral pathway during viral attack in vivo.
Keyword
antiviral activitypepper (Capsicum annuum L.)PR-10ribonucleolytic activitytobacco mosaic virus
ISSN
0960-7412
Publisher
Wiley
DOI
http://dx.doi.org/10.1046/j.1365-313X.2003.01951.x
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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