Effective production of microinjectable blastocysts for Germ-line transmission of embryonic stem cells

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Title
Effective production of microinjectable blastocysts for Germ-line transmission of embryonic stem cells
Author(s)
Sun-Uk Kim; Ying Hao Han; T H Lee; Byung Hwa Hyun; S H Lee; Dong Seok Lee; Dae Yeul Yu
Bibliographic Citation
Experimental Animals, vol. 53, no. 5, pp. 475-477
Publication Year
2004
Abstract
Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.
Keyword
C57BL/6 miceGerm-line chimeraHost embryo
ISSN
1341-1357
Publisher
Int Press Editing Centre Inc
DOI
http://dx.doi.org/10.1538/expanim.53.475
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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