The coffee diterpene kahweol suppress the inducible nitric oxide synthase expression in macrophages

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Title
The coffee diterpene kahweol suppress the inducible nitric oxide synthase expression in macrophages
Author(s)
J Y Kim; K S Jung; K J Lee; H K Na; Hyo Kon Chun; Yung Hee Kho; H G Jeong
Bibliographic Citation
Cancer Letters, vol. 213, no. 2, pp. 147-154
Publication Year
2004
Abstract
Excessive nitric oxide production by inducible nitric oxide synthase (iNOS) in stimulated inflammatory cells is thought to be a causative factor of cellular injury in cases of inflammation. In recent studies, it has been shown that kahweol, coffee-specific diterpene, exhibit chemoprotective effects. In this study, we investigated the effects of kahweol on the production of and the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. The nitrite production induced by LPS was markedly reduced in a dose-dependent manner. In addition, kahweol suppressed the expression of iNOS protein and iNOS mRNA. Since iNOS transcription has been shown to be under the control of the transcription factor, NF-κB, the effects of kahweol on NF-κB activation were examined. Transient transfection experiments showed that kahweol inhibited NF-κB-dependent transcriptional activity. Moreover, electrophoretic mobility shift assay experiments indicated that kahweol blocked the LPS-induced activation of NF-κB. The results of these studies suggest that the suppression of the transcriptional activation of iNOS by kahweol might be mediated through the inhibition of NF-κB activation. Taken together, the results of our study provide evidence that kahweol possess an anti-inflammatory potential, which constitutes a previously unrecognized biologic activity, and which may provide new insights into the inflammatory process.
Keyword
inducible nitric oxide synthasekahweolmacrophagesnitric oxide
ISSN
0304-3835
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.canlet.2004.04.002
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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