Treatment of genomic DNA with restriction enzyme(s) improves amplification efficiency by polymerase chain reaction = 제한효소 처리된 genomic DNA에 의한 polymerase chain reaction 증폭 효율에 관한 연구

Cited 0 time in scopus
Metadata Downloads
Title
Treatment of genomic DNA with restriction enzyme(s) improves amplification efficiency by polymerase chain reaction = 제한효소 처리된 genomic DNA에 의한 polymerase chain reaction 증폭 효율에 관한 연구
Author(s)
H K Min; Young Hyo Chang
Bibliographic Citation
Korean Journal of Microbiology, vol. 40, no. 3, pp. 254-256
Publication Year
2004
Abstract
Polymerase chain reaction (PCR) is a powerful tool for precisely amplifying selected DNA sequences that have had a broad impact on genomic studies. When examining human α- and β- tryptase genes which have 95% DNA homology, inconsistent PCR amplification of genomic sequences hampered our progress. This study suggests that long PCR technique on the original DNA digested with restriction enzymes improves both efficiency and sensitivity of PCR. These improved results seem to derived from the effective denaturation of the original genomic DNA template or reduction of formation of secondary structures that block either primer annealing or extension in PCR. Elimination of homo- or hetero-duplex products derived from highly homologous genes provides an additional advantage in this study. This communication describes how the use of restriction enzymes improved these efficiencies, and also facilitated studies of highly homologous genes including tryptase genes.
ISSN
0440-2413
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > ABS Research Support Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.