Enhancement of nitric oxide production by corticotropin-releasing hormone (CRH) in murine microglial cells, BV2 = 생쥐 미세아교세포(BV2)에서 corticotropin-releasing hormone (CRH)에 의한 nitric oxide (NO) 생성의 증가

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Title
Enhancement of nitric oxide production by corticotropin-releasing hormone (CRH) in murine microglial cells, BV2 = 생쥐 미세아교세포(BV2)에서 corticotropin-releasing hormone (CRH)에 의한 nitric oxide (NO) 생성의 증가
Author(s)
Y H Yang; Young Yang; D H Cho
Bibliographic Citation
Immune Network, vol. 4, no. 1, pp. 60-64
Publication Year
2004
Abstract
Background: Microglial cells, major immune effector cells in the central nervous system, become activated in neurodegenerative disorders. Activated microglial cells produce proinflammatory mediators such as nitric oxide (NO), tumor necrosis factor-α and interleukin-1β(IL-1β). These proinflammatory mediators have been shown to be significantly increased in the neurodegenerative disorders such as Alzhimer's disease and Pakinson's disease. It was known that one of the neurodegeneration source is stress and it is important to elucidate mechanisms of the stress response for understanding the stress-related disorders and developing improved treatments. Because one of the neuropeptide which plays a main role in regulating the stress response is corticotropinreleasing hormone (CRH), we analyzed the regulation of NO release by CRH in BV2 murine microglial cell as macrophage in the brain. Methods: First, we tested the CRH receptor expression in the mRNA levels by RT-PCR. To test the regulation of NO release by CRH, cells were treated with CRH and then NO release was measured by Griess reagent assay. Results: Our study demonstrated that CRH receptor 1 was expressed in BV2 murine microglial cells and CRH treatment enhanced NO production. Furthermore, additive effects of lipopolysaccaride (LPS) and CRH were confirmed in NO production time dependantly. Conclusion: Taken together, these data indicated that CRH is an important mediator to regulate NO release on microglial cells in the brain during stress.
Keyword
corticotropin-releasing hormonenitric oxidemicroglial cells
ISSN
I000-0135
Publisher
South Korea
DOI
http://dx.doi.org/10.4110/in.2004.4.1.60
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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