Expression and activation of an esterase from Pseudomonas aeruginosa 1001 in Escherichia coli

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dc.contributor.authorJe Hyuk Lee-
dc.contributor.authorSang Ki Rhee-
dc.contributor.authorChul Ho Kim-
dc.date.accessioned2017-04-19T09:01:56Z-
dc.date.available2017-04-19T09:01:56Z-
dc.date.issued2004-
dc.identifier.issn01410229-
dc.identifier.uri10.1016/j.enzmictec.2004.08.029ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6725-
dc.description.abstractAn expression vector was constructed to overproduce a maltose binding protein (MBP)-esterase fusion protein in Escherichia coli. Soluble fusion protein was separated by centrifugation after cell disruption. The fusion protein was partially purified with amylose resin. The higher concentration of fusion protein (above 2 mg/ml) did not show any activity but about 0.3 mg/ml of fusion protein had the highest activity (142 U/ml). It is due to the difficulty of contact between substrate and active site of enzyme in compact form at high concentration. The fusion protein over-expressed could not be separated into MBP and esterase by the action of protease 'Factor Xa'. The esterase could be cleaved from MBP fusion protein by the treatment of SDS with the Factor Xa, and the resulting esterase activity was increased to 34% after cleavage.-
dc.publisherElsevier-
dc.titleExpression and activation of an esterase from Pseudomonas aeruginosa 1001 in Escherichia coli-
dc.title.alternativeExpression and activation of an esterase from Pseudomonas aeruginosa 1001 in Escherichia coli-
dc.typeArticle-
dc.citation.titleEnzyme and Microbial Technology-
dc.citation.number6-
dc.citation.endPage567-
dc.citation.startPage563-
dc.citation.volume35-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.alternativeName이제혁-
dc.contributor.alternativeName이상기-
dc.contributor.alternativeName김철호-
dc.identifier.bibliographicCitationEnzyme and Microbial Technology, vol. 35, no. 6, pp. 563-567-
dc.identifier.doi10.1016/j.enzmictec.2004.08.029-
dc.subject.keywordEsterase-
dc.subject.keywordMaltose binding protein-
dc.subject.keywordPseudomonas aeruginosa-
dc.subject.localEsterase-
dc.subject.localMaltose binding protein-
dc.subject.localPseudomonas aeruginosa-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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