Immunization with virus-like particle of human papillomavirus type 16 L1 elicits CTL immune response in C57BL/6 mice

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Title
Immunization with virus-like particle of human papillomavirus type 16 L1 elicits CTL immune response in C57BL/6 mice
Author(s)
Hyun Mi Pyo; J Y Park; S N Park; H S Kim; Kee Sun ShinHaryoung Poo
Bibliographic Citation
Key Engineering Materials, vol. 277, pp. 119-124
Publication Year
2005
Abstract
The development of a human papillomavirus vaccine has received a lot of recent attention due to the connection between HPV infections and cervical cancer. One promising vaccine to prevent HPV infections is an HPV virus-like particle, and various studies have already shown that HPV VLP immunization elicits a humoral immune response. However, cell mediated immunity is important for the prevention of HPV infections and cancer therapy. Therefore, to check the elicitation of a CTL response by yeast-derived virus- like particles consisting of the HPV 16 capsid protein L1, HPV 16 L1 VLPs were produced and purified, then C57BL/6 mice immunized with the HPV 16 L1 VLPs by subcutaneous injection. Thereafter, the splenocytes from the immunized mice were isolated and a chromium release assay performed using recombinant B16/HPV16 L1cells constantly expressing the HPV16 L1 protein. As a result, the data demonstrated that the T cells from the HPV16 L1 VLP immunized mice exhibited a significantly higher cytotoxic T lymphocyte activity against the recombinant B 16/HPV 16 L1 cells than the T cells from the PBS immunized control mice.
Keyword
cytotoxic T lymphocyte (CTL)human papillomavirus (HPV)lymphoproliferation assayvirus-like particle (VLP)chromium release assay
ISSN
1013-9826
Publisher
Trans Tech Publications
DOI
http://dx.doi.org/10.4028/www.scientific.net/KEM.277-279.119
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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