Paenibacillus elgii sp. nov., with broad antimicrobial activity

Abstract

Two novel spore-forming bacteria with broad antimicrobial activity were isolated from roots of Perilla frutescens. The isolates, SD17T and SD18, were facultatively anaerobic and showed variable Gram reaction. Growth was observed between 20 and 45 °C. DNA G + C content of SD17T was 51.7 mol%, and the major fatty acid was anteiso-C15:0 (54.1%). 16S rRNA gene sequence similarity of SD17T ranged from 98.6 to 91.3% with other Paenibacillus species. The phylogenetic tree showed that isolate SD17T formed a significant monophyletic clade with Paenibacillus koreensis KCTC 2393T and Paenibacillus ehimensis IFO 15659T. DNA-DNA relatedness values for strain SD17T with Paenibacillus koreensis KCTC 2393T and Paenibacillus ehimensis IFO 15659T were 17.4 and 19.8 %, respectively. These isolates thus merit species status within Paenibacillus, for which the name Paenibacillus elgii sp. nov. is proposed. The type strain is SD17T (= KCTC 10016BPT = NBRC 100335T).