Over-expression of human UREB1 in colorectal cancer: HECT domain of human UREB1 inhibits the activity of tumor suppressor p53 protein

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dc.contributor.authorSun Young Yoon-
dc.contributor.authorY H Lee-
dc.contributor.authorJ H Kim-
dc.contributor.authorA S Chung-
dc.contributor.authorJoung Hyuck Joo-
dc.contributor.authorC N Kim-
dc.contributor.authorNam-Soon Kim-
dc.contributor.authorIn Seong Choe-
dc.contributor.authorJae Wha Kim-
dc.date.accessioned2017-04-19T09:01:59Z-
dc.date.available2017-04-19T09:01:59Z-
dc.date.issued2005-
dc.identifier.issn0006-291X-
dc.identifier.uri10.1016/j.bbrc.2004.11.004ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6747-
dc.description.abstractMany fundamental processes, including oncogenesis, have implicated HECT domain proteins with ubiquitin ligase activity. The protein human upstream regulatory element binding protein 1 (hUREB1) is a HECT domain protein whose function is not defined yet. Here, we investigate the function of hUREB1 as a ubiquitin-protein ligase in human colorectal cells. Ectopic expression of the HECT domain of hUREB1 reduces the protein level and transcriptional activity of the p53 tumor suppressor, which is abrogated by the deletion in the HECT domain or point mutations in the essential residues of the HECT domain. The ubiquitination and destabilization of p53 is observed in cells treated with the protease inhibitor MG132, implying that the HECT domain of hUREB1 suppresses the transcriptional activity of p53 through a ubiquitin-dependent degradation pathway. Based on the results of Northern blot analysis, RT-PCR, and immunohistochemical analyses, the over-expression of hUREB1 is associated with colorectal carcinoma. Moreover, protein levels of hUREB1 and p53 were inversely correlated. These findings suggest that hUREB1 can function, at least in part, as a negative regulator of p53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain.-
dc.publisherElsevier-
dc.titleOver-expression of human UREB1 in colorectal cancer: HECT domain of human UREB1 inhibits the activity of tumor suppressor p53 protein-
dc.title.alternativeOver-expression of human UREB1 in colorectal cancer: HECT domain of human UREB1 inhibits the activity of tumor suppressor p53 protein-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number1-
dc.citation.endPage17-
dc.citation.startPage7-
dc.citation.volume326-
dc.contributor.affiliatedAuthorSun Young Yoon-
dc.contributor.affiliatedAuthorJoung Hyuck Joo-
dc.contributor.affiliatedAuthorNam-Soon Kim-
dc.contributor.affiliatedAuthorIn Seong Choe-
dc.contributor.affiliatedAuthorJae Wha Kim-
dc.contributor.alternativeName윤선영-
dc.contributor.alternativeName이영희-
dc.contributor.alternativeName김주헌-
dc.contributor.alternativeName정안식-
dc.contributor.alternativeName주종혁-
dc.contributor.alternativeName김창남-
dc.contributor.alternativeName김남순-
dc.contributor.alternativeName최인성-
dc.contributor.alternativeName김재화-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 326, no. 1, pp. 7-17-
dc.identifier.doi10.1016/j.bbrc.2004.11.004-
dc.subject.keywordColorectal carcinoma-
dc.subject.keywordE3 ubiquitin ligase-
dc.subject.keywordHECT domain-
dc.subject.keywordHuman UREB1-
dc.subject.keywordOver-expression-
dc.subject.keywordp53 destabilization-
dc.subject.keywordp53 transcriptional activity-
dc.subject.localColorectal carcinoma-
dc.subject.localcolorectal carcinoma-
dc.subject.localE3 ubiquitinligase-
dc.subject.localE3 ubiquitin ligase-
dc.subject.localHECT domain-
dc.subject.localHuman UREB1-
dc.subject.localOverexpression-
dc.subject.localoverexpression-
dc.subject.localover-expression-
dc.subject.localOver-expression-
dc.subject.localp53 destabilization-
dc.subject.localp53 transcriptional activity-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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