EGFP-labeled mouse embryonic stem cells for in vivo chasing = 생체내 이식된 배아줄기세포 유래 세포의 추적을 위한 형광표지 배아줄기세포주의 수립

Abstract

It is important to chase the cells injected into animal body for evaluation of cell therapy. We established EGFP labeled mouse embryonic stem cells that could be easily detected by their fluorescence expression in frozen types of cells. They also could form EGFP expressing teratomas when they were injected into NOD-scid mice or nude mice. When these cells were injected into peripheral blood of ICR mice, the cells disappeared within 30 sec from the peripheral blood. The injected cells were found mainly in the lung when injected through tail vain. However, they were evenly distributed onto organs when injected into the left ventricle. These cells could be detected by fluorescence microscopy, flow cytometric analysis or confocal microscopy without any treatment on the cells. Accordingly, the embryonic stem cells labeled with EGFP might be very useful in analyzing cells derived from embryonic stem cells in mixed cell culture in vitro, and also very helpful in analyzing transplanted cells derived from differentiated embryonic stem cells.