Activity staining of glutathione peroxidase after two-dimensional gel electrophoresis

Cited 0 time in scopus
Metadata Downloads
Title
Activity staining of glutathione peroxidase after two-dimensional gel electrophoresis
Author(s)
Chang Won Kho; Sung Goo Park; Do Hee Lee; S Cho; G T Oh; S Kang; Byoung Chul Park
Bibliographic Citation
Molecules and Cells, vol. 18, no. 3, pp. 369-373
Publication Year
2004
Abstract
We have developed a method for rapid activity staining of proteins with glutathione peroxidase (GPx) activity after 2-D gel electrophoresis. After separating proteins extracted from yeast, or mouse red blood cells, by two-dimensional gel electrophoresis, SDS was removed and the gel was submerged in a Tris-HCl buffer containing glutathione and hydrogen peroxide, followed by incubation with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and phenazine methosulfate (PMS). After this proteins with GPx activity appeared as clear zones on a purple background. This relatively simple activity staining method could be useful for rapid screening of proteins with GPx activity in cell extracts.
Keyword
2-D gelactivity stainingglutathioneglutathione peroxidase
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.