DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiwon Ahn | - |
dc.contributor.author | Kyung Sook Chung | - |
dc.contributor.author | Dong Uk Kim | - |
dc.contributor.author | Mi Sun Won | - |
dc.contributor.author | Lila Kim | - |
dc.contributor.author | K S Kim | - |
dc.contributor.author | Miyoung Nam | - |
dc.contributor.author | Shin Jung Choi | - |
dc.contributor.author | Hyoung-Chin Kim | - |
dc.contributor.author | M Yoon | - |
dc.contributor.author | S K Chae | - |
dc.contributor.author | Kwang Lae Hoe | - |
dc.date.accessioned | 2017-04-19T09:02:11Z | - |
dc.date.available | 2017-04-19T09:02:11Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6791 | - |
dc.description.abstract | The hepatitis C virus is associated with the development of liver cirrhosis and hepatocellular carcinomas. Among the 10 polyproteins produced by the virus, no function has been clearly assigned to the non-structural 5A (NS5A) protein. This study was designed to identify the hepatocellular proteins that interact with NS5A of the HCV. Yeast two-hybrid experiments were performed with a human liver cDNA prey-library, using five different NS5A derivatives as baits, the full-length NS5A (NS5A-F, amino acid (aa) 1-447) and its four different derivatives, denoted as NS5A-A (aa 1-150), -B (aa 1-300), -C (aa 300-447) and D (aa 150-447). NS5A-F, NS5A-B and NS5A-C gave two, two and 10 candidate clones, respectively, including an AHNAK-related protein, the secreted frizzled-related protein 4 (SFRP4), the N-myc downstream regulated gene 1 (NDRG1), the cellular retinoic acid binding protein 1 (CRABP-1), ferritin heavy chain (FTH1), translokin, tumor-associated calcium signal transducer 2 (TACSTD2), phosphatidylinositol 4-kinase (PI4K) and centaurinδ 2 (CENTδ2). However, NS5A-A produced no candidates and NS5A-D was not suitable as bait due to transcriptional activity. Based on an in vitro binding assay, CRABP-1, PI4K, CENTδ2 and two unknown fusion proteins with maltose binding protein (MBP), were confirmed to interact with the glutathione S-transferase (GST)/NS5A fusion protein. Furthermore, the interactions of CRABP-1, PI4K and CENTδ2 were not related to the PXXP motif (class II), as judged by a domain analysis. While their biological relevance is under investigation, the results contribute to a better understanding of the possible role of NS5A in hepatocellular signaling pathways. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Systematic identification of hepatocellular proteins interacting with NS5A of the hepatitis C virus | - |
dc.title.alternative | Systematic identification of hepatocellular proteins interacting with NS5A of the hepatitis C virus | - |
dc.type | Article | - |
dc.citation.title | BMB Reports | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 748 | - |
dc.citation.startPage | 741 | - |
dc.citation.volume | 37 | - |
dc.contributor.affiliatedAuthor | Jiwon Ahn | - |
dc.contributor.affiliatedAuthor | Kyung Sook Chung | - |
dc.contributor.affiliatedAuthor | Dong Uk Kim | - |
dc.contributor.affiliatedAuthor | Mi Sun Won | - |
dc.contributor.affiliatedAuthor | Lila Kim | - |
dc.contributor.affiliatedAuthor | Miyoung Nam | - |
dc.contributor.affiliatedAuthor | Shin Jung Choi | - |
dc.contributor.affiliatedAuthor | Hyoung-Chin Kim | - |
dc.contributor.affiliatedAuthor | Kwang Lae Hoe | - |
dc.contributor.alternativeName | 안지원 | - |
dc.contributor.alternativeName | 정경숙 | - |
dc.contributor.alternativeName | 김동욱 | - |
dc.contributor.alternativeName | 원미선 | - |
dc.contributor.alternativeName | 김리라 | - |
dc.contributor.alternativeName | 김경신 | - |
dc.contributor.alternativeName | 남미영 | - |
dc.contributor.alternativeName | 최신정 | - |
dc.contributor.alternativeName | 김형진 | - |
dc.contributor.alternativeName | 윤미정 | - |
dc.contributor.alternativeName | 채선기 | - |
dc.contributor.alternativeName | 허광래 | - |
dc.identifier.bibliographicCitation | BMB Reports, vol. 37, no. 6, pp. 741-748 | - |
dc.identifier.doi | 10.5483/bmbrep.2004.37.6.741 | - |
dc.subject.keyword | HCV | - |
dc.subject.keyword | NS5A | - |
dc.subject.keyword | PXXP domain | - |
dc.subject.keyword | yeast two-hybrid | - |
dc.subject.local | HCV | - |
dc.subject.local | NS5A | - |
dc.subject.local | PXXP domain | - |
dc.subject.local | Yeast two-hybrid | - |
dc.subject.local | yeast two-hybrid | - |
dc.subject.local | Yeast two hybrid | - |
dc.description.journalClass | Y | - |
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