Systematic identification of hepatocellular proteins interacting with NS5A of the hepatitis C virus

Cited 52 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJiwon Ahn-
dc.contributor.authorKyung Sook Chung-
dc.contributor.authorDong Uk Kim-
dc.contributor.authorMi Sun Won-
dc.contributor.authorLila Kim-
dc.contributor.authorK S Kim-
dc.contributor.authorMiyoung Nam-
dc.contributor.authorShin Jung Choi-
dc.contributor.authorHyoung-Chin Kim-
dc.contributor.authorM Yoon-
dc.contributor.authorS K Chae-
dc.contributor.authorKwang Lae Hoe-
dc.date.accessioned2017-04-19T09:02:11Z-
dc.date.available2017-04-19T09:02:11Z-
dc.date.issued2004-
dc.identifier.issn12258687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6791-
dc.description.abstractThe hepatitis C virus is associated with the development of liver cirrhosis and hepatocellular carcinomas. Among the 10 polyproteins produced by the virus, no function has been clearly assigned to the non-structural 5A (NS5A) protein. This study was designed to identify the hepatocellular proteins that interact with NS5A of the HCV. Yeast two-hybrid experiments were performed with a human liver cDNA prey-library, using five different NS5A derivatives as baits, the full-length NS5A (NS5A-F, amino acid (aa) 1-447) and its four different derivatives, denoted as NS5A-A (aa 1-150), -B (aa 1-300), -C (aa 300-447) and D (aa 150-447). NS5A-F, NS5A-B and NS5A-C gave two, two and 10 candidate clones, respectively, including an AHNAK-related protein, the secreted frizzled-related protein 4 (SFRP4), the N-myc downstream regulated gene 1 (NDRG1), the cellular retinoic acid binding protein 1 (CRABP-1), ferritin heavy chain (FTH1), translokin, tumor-associated calcium signal transducer 2 (TACSTD2), phosphatidylinositol 4-kinase (PI4K) and centaurinδ 2 (CENTδ2). However, NS5A-A produced no candidates and NS5A-D was not suitable as bait due to transcriptional activity. Based on an in vitro binding assay, CRABP-1, PI4K, CENTδ2 and two unknown fusion proteins with maltose binding protein (MBP), were confirmed to interact with the glutathione S-transferase (GST)/NS5A fusion protein. Furthermore, the interactions of CRABP-1, PI4K and CENTδ2 were not related to the PXXP motif (class II), as judged by a domain analysis. While their biological relevance is under investigation, the results contribute to a better understanding of the possible role of NS5A in hepatocellular signaling pathways.-
dc.publisherSouth Korea-
dc.titleSystematic identification of hepatocellular proteins interacting with NS5A of the hepatitis C virus-
dc.title.alternativeSystematic identification of hepatocellular proteins interacting with NS5A of the hepatitis C virus-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number6-
dc.citation.endPage748-
dc.citation.startPage741-
dc.citation.volume37-
dc.contributor.affiliatedAuthorJiwon Ahn-
dc.contributor.affiliatedAuthorKyung Sook Chung-
dc.contributor.affiliatedAuthorDong Uk Kim-
dc.contributor.affiliatedAuthorMi Sun Won-
dc.contributor.affiliatedAuthorHyoung-Chin Kim-
dc.contributor.alternativeName안지원-
dc.contributor.alternativeName정경숙-
dc.contributor.alternativeName김동욱-
dc.contributor.alternativeName원미선-
dc.contributor.alternativeName김리라-
dc.contributor.alternativeName김경신-
dc.contributor.alternativeName남미영-
dc.contributor.alternativeName최신정-
dc.contributor.alternativeName김형진-
dc.contributor.alternativeName윤미정-
dc.contributor.alternativeName채선기-
dc.contributor.alternativeName허광래-
dc.identifier.bibliographicCitationBMB Reports, vol. 37, no. 6, pp. 741-748-
dc.identifier.doi10.5483/bmbrep.2004.37.6.741-
dc.subject.keywordHCV-
dc.subject.keywordNS5A-
dc.subject.keywordPXXP domain-
dc.subject.keywordyeast two-hybrid-
dc.subject.localHCV-
dc.subject.localNS5A-
dc.subject.localPXXP domain-
dc.subject.localyeast two-hybrid-
dc.subject.localYeast two hybrid-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.