Bax-induced cell death of Arabidopsis is meditated through reactive oxygen-dependent and -independent processes

Cited 70 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorD Baek-
dc.contributor.authorJ Nam-
dc.contributor.authorY D Koo-
dc.contributor.authorD H Kim-
dc.contributor.authorJiyoung Lee-
dc.contributor.authorJae Cheol Jeong-
dc.contributor.authorSang Soo Kwak-
dc.contributor.authorW S Chung-
dc.contributor.authorC O Lim-
dc.contributor.authorJ D Bahk-
dc.contributor.authorJ C Hong-
dc.contributor.authorS Y Lee-
dc.contributor.authorM Kawai-Yamada-
dc.contributor.authorH Uchimiya-
dc.contributor.authorD J Yun-
dc.date.accessioned2017-04-19T09:02:11Z-
dc.date.available2017-04-19T09:02:11Z-
dc.date.issued2004-
dc.identifier.issn0167-4412-
dc.identifier.uri10.1007/s11103-004-3096-4ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6792-
dc.description.abstractAn Arabidopsis protoplast system was developed for dissecting plant cell death in individual cells. Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces apoptotic-like cell death in Arabidopsis. Bax accumulation in Arabidopsis mesophyll protoplasts expressing murine Bax cDNA from a glucocorticoid-inducible promoter results in cytological characteristics of apoptosis, namely DNA fragmentation, increased vacuolation, and loss of plasma membrane integrity. In vivo targeting analysis monitored using jellyfish green fluorescent protein (GFP) reporter indicated full-length Bax was localized to the mitochondria, as it does in animal cells. Deletion of the carboxyl-terminal transmembrane domain of Bax completely abolished targeting to mitochondria. Bax expression was followed by reactive oxygen species (ROS) accumulation. Treatment of protoplasts with the antioxidant N-acetyl-L-cysteine (NAC) during induction of Bax expression strongly suppressed Bax-mediated ROS production and the cell death phenotype. However, some population of the ROS depleted cells still induced cell death, indicating that there is a process that Bax-mediated plant cell death is independent of ROS accumulation. Accordingly, suppression of Bax-mediated plant cell death also takes place in two different processes. Over-expression of a key redox-regulator, Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) down-regulated ROS accumulation and suppressed Bax-mediated cell death and transient expression of Arabidopsis Bax inhibitor-1 (AtBI-1) substantially suppressed Bax-induced cell death without altering cellular ROS level. Taken together, our results collectively suggest that the Bax-mediated cell death and its suppression in plants is mediated by ROS-dependent and -independent processes.-
dc.publisherSpringer-
dc.titleBax-induced cell death of Arabidopsis is meditated through reactive oxygen-dependent and -independent processes-
dc.title.alternativeBax-induced cell death of Arabidopsisis meditated through reactive oxygen-dependent and -independent processes-
dc.typeArticle-
dc.citation.titlePlant Molecular Biology-
dc.citation.number1-
dc.citation.endPage27-
dc.citation.startPage15-
dc.citation.volume56-
dc.contributor.affiliatedAuthorJiyoung Lee-
dc.contributor.affiliatedAuthorJae Cheol Jeong-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.affiliatedAuthorD J Yun-
dc.contributor.alternativeName백동원-
dc.contributor.alternativeName남재성-
dc.contributor.alternativeName구윤덕-
dc.contributor.alternativeName김도훈-
dc.contributor.alternativeName이지영-
dc.contributor.alternativeName정재철-
dc.contributor.alternativeName곽상수-
dc.contributor.alternativeName정우식-
dc.contributor.alternativeName임채오-
dc.contributor.alternativeName박정동-
dc.contributor.alternativeName홍종찬-
dc.contributor.alternativeName이상열-
dc.contributor.alternativeNameKawai-Yamada-
dc.contributor.alternativeNameUchimiya-
dc.contributor.alternativeName윤대진-
dc.identifier.bibliographicCitationPlant Molecular Biology, vol. 56, no. 1, pp. 15-27-
dc.identifier.doi10.1007/s11103-004-3096-4-
dc.subject.keywordApoptosis-
dc.subject.keywordArabidopsis protoplasts-
dc.subject.keywordBax-
dc.subject.keywordCell death-
dc.subject.keywordMitochondria-
dc.subject.keywordReactive oxygen species-
dc.subject.localapoptosis-
dc.subject.localApoptosis-
dc.subject.localArabidopsis protoplasts-
dc.subject.localBax-
dc.subject.localbax-
dc.subject.localcell death-
dc.subject.localCell death-
dc.subject.localMitochondria-
dc.subject.localmitochondria-
dc.subject.localReactive oxidative species-
dc.subject.localReactive oxygen species(ROS)-
dc.subject.localReactive oxygen species-
dc.subject.localReactive Oxygen Species (ROS)-
dc.subject.localReactive Oxygen Species-
dc.subject.localROS-
dc.subject.localReactive oxygen species (ROS)-
dc.subject.localreactive oxygen species-
dc.subject.localreactive oxygen species (ROS)-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.