Development of transgenic soybean using Agrobacterium tumefaciens = Agrobacterium tumefaciens을 이용한 대두 형질전환체 개발

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Title
Development of transgenic soybean using Agrobacterium tumefaciens = Agrobacterium tumefaciens을 이용한 대두 형질전환체 개발
Author(s)
M A Cho; D W Choi; Jang Ryol Liu; T Clemente; P S Choi
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 31, no. 4, pp. 255-259
Publication Year
2004
Abstract
Agrobacterium tumefaciens-mediated cotyledonary node transformation was used to produce transgenic soybean. Cotyledonary node explants of three cultivars and one genotype were co-cultivated with strains Agrobacterium (LBA4404, GV3101, EHA101, C58) containing the binary vectors (pCAMBIA3301 and pPTN289) carrying with CaMV 35S promoter-GUS gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depend on bacteria strain. The EHA101 strain of the bacterial strains employed gave the maximum efficiency (3.6%). One hundred-six lines transformed showed the resistance in glufosinate. Histochemical GUS assay showed that at least 11 plants transformed with the GUS gene were positive response. The soybean transformants were obtained from the Thorne (5 plants), 1049 (5 plants) and Bakun (1 plant), respectively. Southern blot analysis and leaf painting assay revealed that the GUS and bar gene segregated and expressed in their progeny.
Keyword
agrobacterium strainsβ-glucuoinidase (GUS)glycine max L.transformation
ISSN
1015-5880
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2004.31.4.255
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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