DC Field | Value | Language |
---|---|---|
dc.contributor.author | D W Choi | - |
dc.contributor.author | J D Jung | - |
dc.contributor.author | Y I Ha | - |
dc.contributor.author | H W Park | - |
dc.contributor.author | D S In | - |
dc.contributor.author | Hwa-Jee Chung | - |
dc.contributor.author | Jang Ryol Liu | - |
dc.date.accessioned | 2017-04-19T09:02:16Z | - |
dc.date.available | 2017-04-19T09:02:16Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0721-7714 | - |
dc.identifier.uri | 10.1007/s00299-004-0845-4 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6807 | - |
dc.description.abstract | Methyl jasmonate (MeJA) treatment increases the levels of plant secondary metabolites, including ginsenosides, which are considered to be the main active compounds in ginseng (Panax ginseng C.A. Meyer). To create a ginseng gene resource that contains the genes involved in the biosynthesis of secondary metabolites, including ginsenosides, we generated 3,134 expression sequence tags (ESTs) from MeJA-treated ginseng hairy roots. These ESTs assembled into 370 clusters and 1,680 singletons. Genes yielding highly abundant transcripts were those encoding proteins involved in fatty acid desaturation, the defense response, and the biosynthesis of secondary metabolites. Analysis of the latter group revealed a number of genes that may be involved in the biosynthesis of ginsenosides, namely, oxidosqualene cyclase (OSC), cytochrome P450, and glycosyltransferase. A novel OSC gene was also identified by this analysis. RNA gel blot analysis confirmed that transcription of this OSC gene, along with squalene synthase (SS) and squalene epoxidase (SE) gene transcription, is increased by MeJA treatment. This ginseng EST data set will also provide important information on the genes that are involved in the biosynthesis of other secondary metabolites and the genes that are responsive to MeJA treatment. | - |
dc.publisher | Springer | - |
dc.title | Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites | - |
dc.title.alternative | Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites | - |
dc.type | Article | - |
dc.citation.title | Plant Cell Reports | - |
dc.citation.number | 8 | - |
dc.citation.endPage | 566 | - |
dc.citation.startPage | 557 | - |
dc.citation.volume | 23 | - |
dc.contributor.affiliatedAuthor | Hwa-Jee Chung | - |
dc.contributor.affiliatedAuthor | Jang Ryol Liu | - |
dc.contributor.alternativeName | 최동욱 | - |
dc.contributor.alternativeName | 정종덕 | - |
dc.contributor.alternativeName | 하영임 | - |
dc.contributor.alternativeName | 박현우 | - |
dc.contributor.alternativeName | 인동수 | - |
dc.contributor.alternativeName | 정화지 | - |
dc.contributor.alternativeName | 유장렬 | - |
dc.identifier.bibliographicCitation | Plant Cell Reports, vol. 23, no. 8, pp. 557-566 | - |
dc.identifier.doi | 10.1007/s00299-004-0845-4 | - |
dc.subject.keyword | Expression sequence tags | - |
dc.subject.keyword | Ginsenoside biosynthesis | - |
dc.subject.keyword | Methyl jasmonate | - |
dc.subject.keyword | Panax ginseng | - |
dc.subject.keyword | Secondary metabolism | - |
dc.subject.local | Expression sequence tags | - |
dc.subject.local | Expression sequence tags (EST) | - |
dc.subject.local | Ginsenoside biosynthesis | - |
dc.subject.local | methyl jasmonate | - |
dc.subject.local | Methyl jasmonate (MeJA) | - |
dc.subject.local | Methyl jasmonate | - |
dc.subject.local | panax ginseng | - |
dc.subject.local | Panax ginseng | - |
dc.subject.local | secondary metabolism | - |
dc.subject.local | Secondary metabolism | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.