DC Field | Value | Language |
---|---|---|
dc.contributor.author | J I Jun | - |
dc.contributor.author | C W Chung | - |
dc.contributor.author | H J Lee | - |
dc.contributor.author | J O Pyo | - |
dc.contributor.author | Kee Nyung Lee | - |
dc.contributor.author | Nam-Soon Kim | - |
dc.contributor.author | Yong Sung Kim | - |
dc.contributor.author | Hyang Sook Yoo | - |
dc.contributor.author | T H Lee | - |
dc.contributor.author | E Kim | - |
dc.contributor.author | Y K Jung | - |
dc.date.accessioned | 2017-04-19T09:02:18Z | - |
dc.date.available | 2017-04-19T09:02:18Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.uri | 10.1038/sj.onc.1208186 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6822 | - |
dc.description.abstract | Caspase-8 is the most receptor-proximal, upstream caspase in the caspase cascade and plays a key role in cell death triggered by various death receptors. Here, we addressed the role of endogenous caspase-8 in tumor necrosis factor (TNF)-α-induced activation of NF-κB. Direct targeting of caspase-8 with siRNA and antisense (AS) approaches abolished TNF-α-induced activation of NF-κB in NIH3T3, HeLa, and HEK293 cells as determined with luciferase reporter gene and cell fractionation assays. Reconstitution of caspase-8-deficient C33A cells with processing-defective (P/D) mutant of caspase-8 sensitized the cells to TNF-α for NF-κB activation. In contrast to wild-type caspase-8, death effector domain mutant replacing Asp73 with Ala (caspase-8 (D73A)) failed to activate NF-κB and to bind FLICE-associated huge protein (FLASH) in vitro and in vivo. Instead, caspase-8 (D73A) mutant bound to caspase-8 and blocked NF-κB activation triggered by TNF-α and caspase-8. In addition, expression of an NF-κB-activating domain-deletion mutant of FLASH or transfection of FLASH AS oligonucleotides abolished TNF-α and caspase-8, but not phorbol 12-myristate 13-acetate, -induced activation of NF-κB. Further, immunoprecipitation assays showed that caspase-8 formed triple complex with TRAF2 and FLASH. Taken together, these results suggest that endogenous caspase-8 mediates TNF-α-induced activation of NF-κB via FLASH. | - |
dc.publisher | Springer-Nature Pub Group | - |
dc.title | Role of FLASH in caspase-8-mediated activation of NF-κB: dominant-negative function of FLASH mutant in NF-κB signaling pathway | - |
dc.title.alternative | Role of FLASH in caspase-8-mediated activation of NF-κB: dominant-negative function of FLASH mutant in NF-κB signaling pathway | - |
dc.type | Article | - |
dc.citation.title | Oncogene | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 696 | - |
dc.citation.startPage | 688 | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | Kee Nyung Lee | - |
dc.contributor.affiliatedAuthor | Nam-Soon Kim | - |
dc.contributor.affiliatedAuthor | Yong Sung Kim | - |
dc.contributor.affiliatedAuthor | Hyang Sook Yoo | - |
dc.contributor.alternativeName | 전준일 | - |
dc.contributor.alternativeName | 정철웅 | - |
dc.contributor.alternativeName | 이호준 | - |
dc.contributor.alternativeName | 표종옥 | - |
dc.contributor.alternativeName | 이기녕 | - |
dc.contributor.alternativeName | 김남순 | - |
dc.contributor.alternativeName | 김용성 | - |
dc.contributor.alternativeName | 유향숙 | - |
dc.contributor.alternativeName | 이태호 | - |
dc.contributor.alternativeName | 김은희 | - |
dc.contributor.alternativeName | 정용근 | - |
dc.identifier.bibliographicCitation | Oncogene, vol. 24, no. 4, pp. 688-696 | - |
dc.identifier.doi | 10.1038/sj.onc.1208186 | - |
dc.subject.keyword | Caspase-8 | - |
dc.subject.keyword | Dominant negative | - |
dc.subject.keyword | FLASH | - |
dc.subject.keyword | NF-κB | - |
dc.subject.keyword | SiRNA | - |
dc.subject.local | Caspase-8 | - |
dc.subject.local | Dominant negative | - |
dc.subject.local | FLASH | - |
dc.subject.local | Nuclear factor-kappa B | - |
dc.subject.local | nuclear factor κB | - |
dc.subject.local | Nf-κb | - |
dc.subject.local | NF-kB | - |
dc.subject.local | nuclear factor kappa B | - |
dc.subject.local | NF-κB (nuclear factor kappa-B) | - |
dc.subject.local | NF-kappaB | - |
dc.subject.local | Nuclear factor-κb | - |
dc.subject.local | NF-κ B | - |
dc.subject.local | NF-κB | - |
dc.subject.local | NF-kappa B | - |
dc.subject.local | Nuclear factor κB (NF-κB) | - |
dc.subject.local | Nuclear factor κB | - |
dc.subject.local | NFκB | - |
dc.subject.local | Nf-κB | - |
dc.subject.local | Nuclear factor-κB | - |
dc.subject.local | nuclear factorκB | - |
dc.subject.local | Nuclear factor (NF)-κB | - |
dc.subject.local | Nuclear factor kappa B | - |
dc.subject.local | nuclear factor-κB | - |
dc.subject.local | NF-ΚB | - |
dc.subject.local | Nuclear factor-kappa B (NF-κB) | - |
dc.subject.local | Nuclear factor-kappaB | - |
dc.subject.local | nuclear factor-kappaB | - |
dc.subject.local | nuclear factor-kappaB (NF-κB) | - |
dc.subject.local | NFkappaB | - |
dc.subject.local | Nuclear factor kappaB | - |
dc.subject.local | siRNA | - |
dc.subject.local | SiRNA | - |
dc.description.journalClass | Y | - |
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