IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity

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Title
IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity
Author(s)
J Hue; A Kim; H Song; In Pyo Choi; H Park; T Kim; W J Lee; H Kang; D Cho
Bibliographic Citation
Immunology Letters, vol. 96, no. 2, pp. 211-217
Publication Year
2005
Abstract
It has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-l-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells.
Keyword
IL-18Melanomap38 MAPKROISCF
ISSN
0165-2478
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.imlet.2004.08.008
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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